DOI: 10.1101/485888Dec 4, 2018Paper

Bioinformatic analysis of long-lasting transcriptional and translational changes in the basolateral amygdala following acute stress

BioRxiv : the Preprint Server for Biology
Stephanie E SillivanCourtney A Miller

Abstract

Stress profoundly impacts the brain and increases the risk of developing a psychiatric disorder. The brain's response to stress is mediated by a number of pathways that affect gene expression and protein function throughout the cell. Understanding how stress achieves such dramatic effects on the brain requires an understanding of the brain's stress response pathways. The majority of studies focused on molecular changes have employed repeated or chronic stress paradigms to assess the long-term consequences of stress and have not taken an integrative genomic and/or proteomic approach. Here, we determined the lasting impact of a single stressful event (restraint) on the broad molecular profile of the basolateral amygdala complex (BLC), a key brain region mediating emotion, memory and stress. Molecular profiling performed thirty days post-restraint consisted of small RNA sequencing, RNA sequencing and quantitative mass spectrometry and identified long-lasting changes in microRNA (miRNA), messenger RNA (mRNA) and proteins. Alignment of the three datasets further delineated the regulation of stress-specific pathways which were validated by qPCR and Western Blot analysis. From this analysis, mir-29a-5p was identified as a putative reg...Continue Reading

Related Concepts

Amygdaloid Structure
Mental Disorders
Western Blotting
Brain
Emotions
Gene Expression
Genome
Memory
RNA, Messenger
Small Nuclear RNA

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