Bioinformatic identification of euploid and aneuploid embryo secretome signatures in IVF culture media based on MALDI-ToF mass spectrometry.

Journal of Assisted Reproduction and Genetics
Ricardo J PaisRay K Iles

Abstract

Embryo genotyping in IVF clinics aims to identify aneuploid embryos, and current methodologies rely on costly, invasive and time-consuming approaches such as PGT-A screening. MALDI-ToF-based mass spectral analysis of embryo culture has been demonstrated to be a non-invasive, affordable and accurate technique that is able to capture secretome profiles from embryo culture media extremely quick. Thus, aneuploid embryo genotypes can be distinguished from euploids from these profiles towards the development of novel embryo selection tools. A retrospective cohort study, including 292 spent media samples from embryo cultures collected from a single IVF clinic in USA. There were 149 euploid and 165 aneuploid embryos previously analysed by PGT-A next-generation sequencing techniques. Secretome mass spectra of embryos were generated using MALDI-ToF mass spectrometry in the UK. Data was systematically analysed using a fully automated and ultra-fast bioinformatic pipeline developed for the identification of mass spectral signatures. Distinct spectral patterns were found for euploid and aneuploid genotypes in embryo culture media. We identified 12 characteristic peak signatures for euploid and 17 for aneuploid embryos. Data analysis also re...Continue Reading

References

Jan 1, 1993·Journal of Assisted Reproduction and Genetics·J ConaghanH J Leese
Mar 23, 2013·Reproductive Biomedicine Online·Alison CampbellCristina Fontes Lindemann Hickman
Oct 15, 2013·Omics : a Journal of Integrative Biology·Anna Louise SwanJaume Bacardit
Mar 5, 2016·BioMed Research International·Danilo CimadomoLaura Rienzi
Jun 15, 2016·Journal of Assisted Reproduction and Genetics·Paul R BrezinaWilliam G Kearns
Oct 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Juanjuan XuSijia Lu
Jul 13, 2017·Fertility and Sterility·Richard J Paulson
Jan 9, 2019·Journal of Assisted Reproduction and Genetics·Kemal OzgurKevin Coetzee
Jan 27, 2019·Fertility and Sterility·Bo LiangZi-Jiang Chen
May 17, 2019·Journal of Assisted Reproduction and Genetics·Ray K IlesStephen A Butler
Jun 27, 2019·Journal of Assisted Reproduction and Genetics·Pasquale PatrizioNorbert Gleicher

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Citations

Apr 4, 2021·International Journal of Molecular Sciences·Raminta ZmuidinaiteRay K Iles

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