Bioinformatic screening of human ESTs for differentially expressed genes in normal and tumor tissues.

BMC Genomics
Abdel AouacheriaChristian Gautier

Abstract

Owing to the explosion of information generated by human genomics, analysis of publicly available databases can help identify potential candidate genes relevant to the cancerous phenotype. The aim of this study was to scan for such genes by whole-genome in silico subtraction using Expressed Sequence Tag (EST) data. Genes differentially expressed in normal versus tumor tissues were identified using a computer-based differential display strategy. Bcl-xL, an anti-apoptotic member of the Bcl-2 family, was selected for confirmation by western blot analysis. Our genome-wide expression analysis identified a set of genes whose differential expression may be attributed to the genetic alterations associated with tumor formation and malignant growth. We propose complete lists of genes that may serve as targets for projects seeking novel candidates for cancer diagnosis and therapy. Our validation result showed increased protein levels of Bcl-xL in two different liver cancer specimens compared to normal liver. Notably, our EST-based data mining procedure indicated that most of the changes in gene expression observed in cancer cells corresponded to gene inactivation patterns. Chromosomes and chromosomal regions most frequently associated wit...Continue Reading

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Citations

Dec 3, 2008·Pathology Oncology Research : POR·Yan LiuGuancheng Li
Sep 23, 2014·Genetics Research International·Eugenia PoliakovIgor B Rogozin
Feb 5, 2010·Journal of Cancer Research and Clinical Oncology·Yan LiuGuancheng Li
Aug 9, 2007·Molecular Cancer·David MurrayAlan C Moss
Mar 12, 2008·Yi chuan = Hereditas·Yuan LiuQiang-Song Tong

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Methods Mentioned

BETA
nucleic
using

Software Mentioned

ACNUC
UNIGENE
SAGE Genie
R
SAGE
PostgreSQL
eVOC
Ensembl
GeMCore
Java

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