Bioinformatics analysis of candidate proteins Omp2b, P39 and BLS for Brucella multivalent epitope vaccines.

Microbial Pathogenesis
Tong ShaJianbing Ding

Abstract

This study focuses on analyzing the physicochemical properties, structural characteristics and dominant epitopes of Brucella outer membrane protein 2b (Omp2b), periplasmic binding protein (P39) and Brucella lumazine synthase (BLS) proteins by bioinformatics methods, and to provide a theoretical basis for constructing multi-epitope vaccines. The amino acid sequences of three kinds of proteins were obtained from the UniProt database. The highest frequency alleles in northern China were obtained from the AlleleFrequencies database. Analysis of the physicochemical properties of the proteins by ProtParam online software. Analysis of the secondary structure of the proteins were predicted by SOMPA online software. Using SWISS-MODEL online software constructed and analyzed the tertiary structure of the proteins. Using ABCpred, BepiPred, BCPred and SVMTrip online software analyzed linear B cell epitopes of proteins, The T cell dominant epitope of the protein was analyzed using SYFPEITHI, RANKPEP and IEDB online software. Omp2b was identified three linear B cell dominant epitopes, five CD8+ T cell dominant epitopes, and three CD4+ T cell dominant epitopes. P39 was identified three linear B cell dominant epitopes, two CD8+ T cell dominant...Continue Reading

References

Nov 5, 2002·Veterinary Microbiology·Axel CloeckaertPhilip H Elzer
Feb 12, 2009·Journal of Biosciences·Basappa G Mantur, Satish K Amarnath
Jul 17, 2009·Veterinary Microbiology·Mohamed N SeleemNammalwar Sriranganathan
Dec 8, 2010·Bioinformatics·Pascal BenkertTorsten Schwede
Jul 19, 2013·BioMed Research International·Eric Daniel Avila-CalderónAraceli Contreras-Rodríguez
Oct 20, 2015·Omics : a Journal of Integrative Biology·Udayakumar S VishnuJeyaprakash Rajendhran
Feb 13, 2016·Clinical and Experimental Vaccine Research·Maryam GolshaniSaeid Bouzari
Dec 7, 2016·Research in Pharmaceutical Sciences·Soheil YousefiMohammad Hadi Sekhavati
Sep 2, 2017·Journal of Veterinary Science·Jonathan Lalsiamthara, John Hwa Lee
Feb 16, 2018·Journal of Immunology Research·Jose L Sanchez-TrincadoPedro A Reche
May 23, 2018·Nucleic Acids Research·Andrew WaterhouseTorsten Schwede
Jul 10, 2018·Infectious Disorders Drug Targets·Marzieh RezaeiHamid Abtahi
Apr 26, 2019·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Xiao ZhaoJianbing Ding
Jun 10, 2019·Vaccine·Huanhuan HouQisheng Peng
Jul 29, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Melody VatankhahMehdi Mahdavi

❮ Previous
Next ❯

Citations

Jun 5, 2021·Journal of Biomedical Nanotechnology·Yujiao LiJianbing Ding
Aug 31, 2021·Frontiers in Immunology·Mingkai YuJianbing Ding

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.