Bioinformatics analysis of single and multi-hybrid epitopes of GRA-1, GRA-4, GRA-6 and GRA-7 proteins to improve DNA vaccine design against Toxoplasma gondii

Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology
Minoo ShaddelMohammad Reza Tabandeh

Abstract

Toxoplasma gondii, is a causative agent of morbidity and mortality in immunocompromised and congenitally-infected individuals. Attempts to construct DNA vaccines against T. gondii using surface proteins are increasing. The dense granule antigens are highly expressed in the acute and chronic phases of T. gondii infection and considered as suitable DNA vaccine candidates to control toxoplasmosis. In the present study, bioinformatics tools and online software were used to predict, analyze and compare the structural, physical and chemical characters and immunogenicity of the GRA-1, GRA-4, GRA-6 and GRA-7 proteins. Sequence alignment results indicated that the GRA-1, GRA-4, GRA-6 and GRA-7 proteins had low similarity. The secondary structure prediction demonstrated that among the four proteins, GRA-1 and GRA-6 had similar secondary structure except for a little discrepancy. Hydrophilicity/hydrophobicity analysis showed multiple hydrophilic regions and some classical high hydrophilic domains for each protein sequence. Immunogenic epitope prediction results demonstrated that the GRA-1 and GRA-4 epitopes were stable and GRA-4 showed the highest degree of antigenicity. Although the GRA-7 epitope had the highest score of immunogenicity, ...Continue Reading

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Citations

Nov 2, 2019·Transactions of the Royal Society of Tropical Medicine and Hygiene·Keivan AdinehbeigiAlireza Zakeri
May 28, 2020·Frontiers in Cellular and Infection Microbiology·Rochelle Haidee D YbañezYoshifumi Nishikawa
Jan 13, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Morteza ShamsHassan Nourmohammadi
Sep 12, 2020·Microbial Pathogenesis·MohammadReza Chaechi NosratiFarzaneh Shakarami
Jul 3, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Ali AsghariFattaneh Montazeri

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