Bioinformatics functional analysis of let-7a, miR-34a, and miR-199a/b reveals novel insights into immune system pathways and cancer hallmarks for hepatocellular carcinoma

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Bangly SolimanMahmoud El Hefnawi

Abstract

Let-7a, miR-34a, and miR-199 a/b have gained a great attention as master regulators for cellular processes. In particular, these three micro-RNAs act as potential onco-suppressors for hepatocellular carcinoma. Bioinformatics can reveal the functionality of these micro-RNAs through target prediction and functional annotation analysis. In the current study, in silico analysis using innovative servers (miRror Suite, DAVID, miRGator V3.0, GeneTrail) has demonstrated the combinatorial and the individual target genes of these micro-RNAs and further explored their roles in hepatocellular carcinoma progression. There were 87 common target messenger RNAs (p ≤ 0.05) that were predicted to be regulated by the three micro-RNAs using miRror 2.0 target prediction tool. In addition, the functional enrichment analysis of these targets that was performed by DAVID functional annotation and REACTOME tools revealed two major immune-related pathways, eight hepatocellular carcinoma hallmarks-linked pathways, and two pathways that mediate interconnected processes between immune system and hepatocellular carcinoma hallmarks. Moreover, protein-protein interaction network for the predicted common targets was obtained by using STRING database. The indivi...Continue Reading

References

May 29, 1996·International Journal of Cancer. Journal International Du Cancer·V TrochonH Lu
Jul 1, 1996·Proceedings of the Society for Experimental Biology and Medicine·X Qian, G P Tuszynski
Apr 30, 1999·Science·S Artavanis-TsakonasR J Lake
Jan 27, 2000·Cell·D Hanahan, R A Weinberg
Aug 10, 2000·Nature Medicine·J Desbarats, M K Newell
Feb 24, 2001·Gastroenterology·W Z MehalI N Crispe
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 1, 2002·Nature Genetics·Snorri S Thorgeirsson, Joe W Grisham
Aug 20, 2002·Nature Genetics·Takeshi SuzukiNeal G Copeland
Sep 24, 2002·Journal of Cell Science·Claire M WellsAnne J Ridley
Sep 26, 2002·FEBS Letters·Yoshihisa FujitaKenichi Harigaya
Jan 10, 2003·Nucleic Acids Research·Mathias KrullEdgar Wingender
Jan 10, 2003·Nucleic Acids Research·V MatysE Wingender
Nov 25, 2003·Oncogene·Peter ListonRobert G Korneluk
Dec 19, 2003·Nucleic Acids Research·Evelyn CamonRolf Apweiler
Dec 19, 2003·Nucleic Acids Research·Minoru KanehisaMasahiro Hattori
May 6, 2004·Experimental and Molecular Pathology·Minalini LakshmanSerge Jothy
Jun 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Daniel R RhodesArul M Chinnaiyan
Jun 26, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ronnie T PoonSheung Tat Fan
Jul 9, 2004·Cellular Signalling·Paul-Joseph Aspuria, Fuyuhiko Tamanoi
Dec 30, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Fikret SahinMichael Torbenson
Feb 22, 2005·PLoS Biology·Julius BrenneckeStephen M Cohen
Apr 5, 2005·Nature Genetics·Azra KrekNikolaus Rajewsky
Sep 22, 2005·Nature Reviews. Immunology·Michael Karin, Florian R Greten
Oct 22, 2005·The Journal of Clinical Investigation·Klara BalintZhao-Jun Liu
Nov 18, 2005·Blood·Kevin G Leong, Aly Karsan
Dec 31, 2005·Nucleic Acids Research·Sam Griffiths-JonesAnton J Enright
Jan 28, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Kohei IshiyamaToshimasa Asahara
Feb 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ayyakannu AyyananCathrin Brisken
Apr 19, 2006·Gastroenterology·Diego F CalvisiSnorri S Thorgeirsson

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Methods Mentioned

BETA
transfection

Software Mentioned

DIANA
DAVID
TargetScan
PicTar
cytoscape
miRGator
hubba
miRtegrate
TRANSPATH
cyto

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