Bioink Composition and Printing Parameters for 3D Modeling Neural Tissue

Cells
Valentina FantiniCristina Cereda

Abstract

Neurodegenerative diseases (NDs) are a broad class of pathologies characterized by the progressive loss of neurons in the central nervous system. The main problem in the study of NDs is the lack of an adequate realistic experimental model to study the pathogenic mechanisms. Induced pluripotent stem cells (iPSCs) partially overcome the problem, with their capability to differentiate into almost every cell types; even so, these cells alone are not sufficient to unveil the mechanisms underlying NDs. 3D bioprinting allows to control the distribution of cells such as neurons, leading to the creation of a realistic in vitro model. In this work, we analyzed two biomaterials: sodium alginate and gelatin, and three different cell types: a neuroblastoma cell line (SH-SY5Y), iPSCs, and neural stem cells. All cells were encapsulated inside the bioink, printed and cultivated for at least seven days; they all presented good viability. We also evaluated the maintenance of the printed shape, opening the possibility to obtain a reliable in vitro neural tissue combining 3D bioprinting and iPSCs technology, optimizing the study of the degenerative processes that are still widely unknown.

References

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May 24, 2018·Molecular Neurodegeneration·Eduarda G Z CentenoAngela Bithell
Dec 14, 2018·International Journal of Molecular Sciences·Matteo BordoniCristina Cereda

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Citations

May 8, 2020·International Journal of Molecular Sciences·Matteo BordoniCristina Cereda
Mar 3, 2020·Frontiers in Bioengineering and Biotechnology·Ruchi SharmaStephanie M Willerth
Nov 18, 2020·Advanced Healthcare Materials·Melissa CadenaSteven A Sloan
Dec 17, 2020·Frontiers in Immunology·Diana CoralloSanja Aveic
Feb 16, 2021·Frontiers in Bioengineering and Biotechnology·Chrysanthi-Maria MoysidouRóisín Meabh Owens
Apr 10, 2021·APL Bioengineering·Danielle WarrenJeremy M Crook
May 1, 2021·Cancers·Colin H QuinnElizabeth A Beierle
May 3, 2021·International Journal of Biological Macromolecules·Balaji MahendiranGopal Shankar Krishnakumar
Jul 6, 2021·International Journal of Biological Macromolecules·Ye LiuJianan Ren

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Methods Mentioned

BETA
biopsies
bioprinting
bioprinter
Assay
confocal microscopy
electrophoresis
biopsy
PCR
transgenic

Software Mentioned

GraphPad
ImageJ
Cellink
Cellink INKREDIBLE +
GraphPad Prism
Slic3r
FreeCAD©

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