Bioinoculants play a significant role in shaping the rhizospheric microbial community: a field study with Cajanus cajan

World Journal of Microbiology & Biotechnology
Richa SharmaShilpi Sharma

Abstract

The present study is an attempt to understand the impact of bioinoculants, Azotobacter chroococcum (A), Bacillus megaterium (B), Pseudomonas fluorescens (P), on (a) soil and plant nutrient status, (b) total resident and active bacterial communities, and (c) genes and transcripts involved in nitrogen cycle, during cultivation of Cajanus cajan. In terms of available macro- and micro-nutrients, triple inoculation of the bioinoculants (ABP) competed well with chemical fertilizer (CF). Their 'non-target' effects were assessed in terms of the abundance and activity of the resident bacterial community by employing denaturing gradient gel electrophoresis (DGGE). The resident bacterial community (16S rRNA gene) was stable, while the active fraction (16S rRNA transcripts) was influenced (in terms of abundance) by the treatments. Quantification of the genes and transcripts involved in N cycle by qPCR revealed an increase in the transcripts of nifH in the soil treated with ABP over CF, with an enhancement of 3.36- and 1.57- fold at flowering and maturity stages of plant growth, respectively. The bioinoculants shaped the resident microflora towards a more beneficial community, which helped in increasing soil N turnover and hence, soil ferti...Continue Reading

References

May 12, 2004·Journal of Microbiological Methods·Juan C López-GutiérrezLaurent Philippot
Jul 21, 2004·Briefings in Bioinformatics·Sudhir KumarMasatoshi Nei
Oct 20, 2005·Environmental Microbiology·Helmut BürgmannJosef Zeyer
Aug 13, 2008·Proceedings of the National Academy of Sciences of the United States of America·Steven D Allison, Jennifer B H Martiny
Apr 4, 2012·Annual Review of Chemical and Biomolecular Engineering·Michael J LiszkaDouglas S Clark
May 11, 2012·Applied Microbiology and Biotechnology·Tae Gwan KimKyung-Suk Cho
Jul 10, 2012·Trends in Biotechnology·Van H T Pham, Jaisoo Kim
Jul 31, 2012·Journal of Microbiological Methods·Shilpi SharmaMichael Schloter
Aug 21, 2013·BioMed Research International·Darine Trabelsi, Ridha Mhamdi
Jun 10, 2015·Journal of Microbiological Methods·Glade DlottStephanie Yarwood
Nov 4, 2015·Frontiers in Microbiology·Heini KutvonenMalin Bomberg
Sep 21, 2017·Frontiers in Microbiology·Luisa W Hugerth, Anders F Andersson

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Methods Mentioned

BETA
electrophoresis
PCR
PCA

Software Mentioned

NGS
MEGA
SPSS
Gel compar
Basic Local Alignment Search Tool ( BLAST )
SPSS Statistics
Gel Compar II
DGGE

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