Bioinspired Design of Alcohol Dehydrogenase@nano TiO₂ Microreactors for Sustainable Cycling of NAD⁺/NADH Coenzyme

Nanomaterials
Sen LinXiaoyu Fan

Abstract

The bioinspired design and construction of enzyme@capsule microreactors with specific cell-like functionality has generated tremendous interest in recent years. Inspired by their fascinating complexity, scientists have endeavored to understand the essential aspects of a natural cell and create biomimicking microreactors so as to immobilize enzymes within the hierarchical structure of a microcapsule. In this study, simultaneous encapsulation of alcohol dehydrogenase (ADH) was achieved during the preparation of microcapsules by the Pickering emulsion method using amphiphilic modified TiO₂ nanoparticles (NPs) as building blocks for assembling the photocatalytic microcapsule membrane. The ADH@TiO₂ NP microreactors exhibited dual catalytic functions, i.e., spatially confined enzymatic catalysis and the membrane-associated photocatalytic oxidation under visible light. The sustainable cycling of nicotinamide adenine dinucleotide (NAD) coenzyme between NADH and NAD⁺ was realized by enzymatic regeneration of NADH from NAD⁺ reduction, and was provided in a form that enabled further photocatalytic oxidation to NAD⁺ under visible light. This bioinspired ADH@TiO₂ NP microreactor allowed the linking of a semiconductor mineral-based inorganic...Continue Reading

References

Dec 5, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Chengli HuoStephen Mann
Mar 31, 2015·Life·David M Miller, Jacqueline M Gulbis
Apr 22, 2015·Chemical Communications : Chem Comm·Kangle LvStephen Mann
Jul 16, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·David P Summers, David Rodoni
Mar 17, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Khrongkhwan AkkarachaneeyakornStephen Mann
May 12, 2016·Chemical Communications : Chem Comm·Wen-Xin MaoLi-Jun Wan
Jan 18, 2017·Accounts of Chemical Research·Bastiaan C Buddingh', Jan C M van Hest
Oct 3, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Jinghui WangZhimin He

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Citations

May 9, 2019·Angewandte Chemie·Calum T J FergusonKai A I Zhang
Jul 11, 2020·Advanced Biosystems·Lado OtrinTanja Vidaković-Koch
Mar 19, 2019·Chembiochem : a European Journal of Chemical Biology·Beatriz C MaKatharina Landfester
Jul 6, 2019·Frontiers in Plant Science·Li TaiKun-Ming Chen
May 11, 2021·Small·Isabella Nymann WestenseeBrigitte Städler
May 10, 2020·ACS Applied Materials & Interfaces·Seong-Min JoKatharina Landfester
Jan 18, 2022·Nanoscale·Julian Heuer, Calum T J Ferguson

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Methods Mentioned

BETA
X-ray

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