Bioinspired liver scaffold design criteria

Organogenesis
Giorgio MatteiArti Ahluwalia

Abstract

Maintaining hepatic functional characteristics in-vitro is considered one of the main challenges in engineering liver tissue. As hepatocytes cultured ex-vivo are deprived of their native extracellular matrix (ECM) milieu, developing scaffolds that mimic the biomechanical and physicochemical properties of the native ECM is thought to be a promising approach for successful tissue engineering and regenerative medicine applications. On the basis that the decellularized liver matrix represents the ideal design template for engineering bioinspired hepatic scaffolds, to derive quantitative descriptors of liver ECM architecture, we characterised decellularised liver matrices in terms of their biochemical, viscoelastic and structural features along with porosity, permeability and wettability. Together, these data provide a unique set of quantitative design criteria which can be used to generate guidelines for fabricating biomaterial scaffolds for liver tissue engineering. As proof-of-concept, we investigated hepatic cell response to substrate viscoelasticity. On collagen hydrogels mimicking decellularised liver mechanics, cells showed superior morphology, higher viability and albumin secretion than on stiffer and less viscous substrates...Continue Reading

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Citations

Feb 11, 2020·Biomechanics and Modeling in Mechanobiology·Adela CapilnasiuDavid Nordsletten
Oct 28, 2019·Frontiers in Bioengineering and Biotechnology·Chiara MagliaroArti Ahluwalia
Oct 19, 2019·Bioengineering·Monique M A VerstegenLuc J W van der Laan
Aug 28, 2021·Antioxidants·Jorge OteroIsaac Almendros

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Methods Mentioned

BETA
GTA
Assay
enzyme-linked immunosorbent assay

Software Mentioned

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ImageJ
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hisTOOLogy

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