Bioinspired tuning of hydrogel permeability-rigidity dependency for 3D cell culture

Scientific Reports
Min Kyung LeeHyunjoon Kong

Abstract

Hydrogels are being extensively used for three-dimensional immobilization and culture of cells in fundamental biological studies, biochemical processes, and clinical treatments. However, it is still a challenge to support viability and regulate phenotypic activities of cells in a structurally stable gel, because the gel becomes less permeable with increasing rigidity. To resolve this challenge, this study demonstrates a unique method to enhance the permeability of a cell-laden hydrogel while avoiding a significant change in rigidity of the gel. Inspired by the grooved skin textures of marine organisms, a hydrogel is assembled to present computationally optimized micro-sized grooves on the surface. Separately, a gel is engineered to preset aligned microchannels similar to a plant's vascular bundles through a uniaxial freeze-drying process. The resulting gel displays significantly increased water diffusivity with reduced changes of gel stiffness, exclusively when the microgrooves and microchannels are aligned together. No significant enhancement of rehydration is achieved when the microgrooves and microchannels are not aligned. Such material design greatly enhances viability and neural differentiation of stem cells and 3D neural ...Continue Reading

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Citations

Jan 10, 2017·Tissue Engineering. Part B, Reviews·Changjiang Fan, Dong-An Wang
Jan 21, 2017·Tissue Engineering. Part B, Reviews·Tanveer Ahmad Mir, Makoto Nakamura
Jan 13, 2019·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Simon C de GrootMargriet A Huisman
Jun 24, 2016·Scientific Reports·Omar YassineJurgen Kosel
Apr 6, 2021·Frontiers in Bioengineering and Biotechnology·Maria Grazia TuponeAnnamaria Cimini
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Dec 9, 2019·ACS Biomaterials Science & Engineering·Heta ThakarAkshay Srivastava

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Methods Mentioned

BETA
nuclear magnetic resonance

Software Mentioned

VNMR
Comsol Multiphysics

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