Abstract
Hydrogels are being extensively used for three-dimensional immobilization and culture of cells in fundamental biological studies, biochemical processes, and clinical treatments. However, it is still a challenge to support viability and regulate phenotypic activities of cells in a structurally stable gel, because the gel becomes less permeable with increasing rigidity. To resolve this challenge, this study demonstrates a unique method to enhance the permeability of a cell-laden hydrogel while avoiding a significant change in rigidity of the gel. Inspired by the grooved skin textures of marine organisms, a hydrogel is assembled to present computationally optimized micro-sized grooves on the surface. Separately, a gel is engineered to preset aligned microchannels similar to a plant's vascular bundles through a uniaxial freeze-drying process. The resulting gel displays significantly increased water diffusivity with reduced changes of gel stiffness, exclusively when the microgrooves and microchannels are aligned together. No significant enhancement of rehydration is achieved when the microgrooves and microchannels are not aligned. Such material design greatly enhances viability and neural differentiation of stem cells and 3D neural ...Continue Reading
References
Jul 9, 1999·Biotechnology and Bioengineering·P Jorjani, S S Ozturk
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·G C KopenD G Phinney
Aug 1, 2000·Experimental Neurology·J Sanchez-RamosP R Sanberg
Nov 17, 2001·Chemical Reviews·K Y Lee, D J Mooney
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·C P HofstetterL Olson
Feb 22, 2002·Journal of Biomedical Materials Research·Jon A Rowley, David J Mooney
Aug 19, 2003·Biomaterials·Jeanie L Drury, David J Mooney
May 5, 2005·Experimental Neurology·P BossolascoE Polli
Nov 19, 2005·Science·Dennis E DischerYu-Li Wang
Jan 1, 1966·Plant Physiology·A E Dimond
Oct 3, 2006·Biomaterials·Ferdinand BrandlAchim Goepferich
May 20, 1996·Biotechnology and Bioengineering·A C JenA G Mikos
Jan 26, 2011·Biomaterials·Chaenyung ChaHyunjoon Kong
Jul 7, 2011·Biotechnology and Bioengineering·Chaenyung ChaHyunjoon Kong
Jul 28, 2011·Journal of Cellular Physiology·Girish PattappaDavid A Lee
May 29, 2014·Nanoscale·Min Kyung Lee, Jonghwi Lee
Jun 6, 2014·Advanced Healthcare Materials·Min Kyung LeeHyunjoon Kong
Citations
Jan 10, 2017·Tissue Engineering. Part B, Reviews·Changjiang Fan, Dong-An Wang
Jan 21, 2017·Tissue Engineering. Part B, Reviews·Tanveer Ahmad Mir, Makoto Nakamura
Jun 27, 2017·Biomacromolecules·Max H RichHyunjoon Kong
Jan 13, 2019·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Simon C de GrootMargriet A Huisman
Feb 18, 2016·Scientific Reports·Irina N SavinaSergey V Mikhalovsky
Jun 24, 2016·Scientific Reports·Omar YassineJurgen Kosel
May 26, 2017·Scientific Reports·Youngsang KoJungmok You
Sep 3, 2020·Royal Society Open Science·Shinya YamahiraToshiyuki Kanamori
Oct 22, 2020·Biofabrication·Jinkyu LeeHeungsoo Shin
Dec 24, 2020·Chemical Communications : Chem Comm·Cameron W EvansK Swaminathan Iyer
Apr 6, 2021·Frontiers in Bioengineering and Biotechnology·Maria Grazia TuponeAnnamaria Cimini
Oct 16, 2020·Tissue Engineering. Part a·Guang YangJohn P Fisher
Oct 11, 2017·Chemical Reviews·Guoyou HuangFeng Xu
Dec 19, 2018·Bioconjugate Chemistry·Mozhdeh ImaninezhadSilviya Petrova Zustiak
Jun 28, 2019·ACS Nano·Hyungjun KimChi Hwan Lee
Dec 9, 2019·ACS Biomaterials Science & Engineering·Heta ThakarAkshay Srivastava