Bioleaching of copper- and zinc-bearing ore using consortia of indigenous iron-oxidizing bacteria

Extremophiles : Life Under Extreme Conditions
Wasim SajjadSuliman Khan

Abstract

Indigenous iron-oxidizing bacteria were isolated on modified selective 9KFe2+ medium from Baiyin copper mine stope, China. Three distinct acidophilic bacteria were isolated and identified by analyzing the sequences of 16S rRNA gene. Based on published sequences of 16S rRNA gene in the GenBank, a phylogenetic tree was constructed. The sequence of isolate WG101 showed 99% homology with Acidithiobacillus ferrooxidans strain AS2. Isolate WG102 exhibited 98% similarity with Leptospirillum ferriphilum strain YSK. Similarly, isolate WG103 showed 98% similarity with Leptospirillum ferrooxidans strain L15. Furthermore, the biotechnological potential of these isolates in consortia form was evaluated to recover copper and zinc from their ore. Under optimized conditions, 77.68 ± 3.55% of copper and 70.58 ± 3.77% of zinc were dissolved. During the bioleaching process, analytical study of pH and oxidation-reduction potential fluctuations were monitored that reflected efficient activity of the bacterial consortia. The FTIR analysis confirmed the variation in bands after treatment with consortia. The impact of consortia on iron speciation within bioleached ore was analyzed using Mössbauer spectroscopy and clear changes in iron speciation was r...Continue Reading

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Jul 15, 2016·Bioresource Technology·Mauricio LatorreAlejandro Maass

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Citations

Feb 29, 2020·Extremophiles : Life Under Extreme Conditions·Jiafeng LiAli Auwalu
Aug 9, 2021·Environmental Geochemistry and Health·Darya EgorovaDenis Kormshchikov
Jan 31, 2020·Journal of Environmental Management·Bahareh ArabJavad Hamedi

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Methods Mentioned

BETA
PCR
X-ray
bacterial treatment

Software Mentioned

BioEdit
GraphPad
MEGA
BLAST
Excel

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