Biolistic expression of the macrophage colony stimulating factor receptor in organotypic cultures induces an inflammatory response

Journal of Neuroscience Research
Olivera M MitrasinovicG M Murphy

Abstract

The receptor for macrophage colony-stimulating factor (M-CSFR; c-fms) is expressed at increased levels by microglia in Alzheimer's disease (AD) and in mouse models for AD. Increased expression of M-CSFR on cultured microglia results in a strong proinflammatory response, but the relevance of this cell culture finding to intact brain is unknown. To determine the effects of increased microglial expression of M-CSFR in a complex organotypic environment, we developed a system for biolistic transfection of microglia in hippocampal slice cultures. The promoter for the Mac-1 integrin alpha subunit CD11b is active in cells of myeloid origin. In the brain, CD11b expression is restricted to microglia. Constructs consisting of the promoter for CD11b and a c-fms cDNA or an enhanced green fluorescent protein (EGFP) cDNA were introduced into monotypic cultures of microglia, neurons, and astrocytes. Strong CD11b promoter activity was observed in microglia, whereas little activity was observed in other cell types. Biolistic transfection of organotypic hippocampal cultures with the CD11b/c-fms construct resulted in expression of the c-fms mRNA and protein that was localized to microglia. Furthermore, biolistic overexpression of M-CSFR on microgl...Continue Reading

References

Sep 1, 1992·Journal of Neuropathology and Experimental Neurology·K WilliamsV W Yong
Apr 1, 1992·Journal of Neuroscience Research·V BocchiniH Kettenmann
Apr 1, 1991·Journal of Neuroscience Methods·L StoppiniD Muller
Nov 1, 1990·Journal of Neuroimmunology·H Akiyama, P L McGeer
May 1, 1990·Journal of Neuroimmunology·E BlasiF Bistoni
Jul 1, 1994·Journal of Neuroscience Research·K WilliamsJ McLaurin
Oct 1, 1996·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·B W Coltman, C F Ide
Jan 1, 1997·Journal of Neuroscience Research·A G Rabchevsky, W J Streit
Nov 5, 1997·Trends in Neurosciences·B H GähwilerS M Thompson
Oct 7, 1998·Progress in Neurobiology·M A Cuadros, J Navascués
Dec 16, 1998·Journal of Neural Transmission. Supplementum·P L McGeer, E G McGeer
Apr 15, 1999·Annual Review of Neuroscience·F González-Scarano, G Baltuch
Jun 22, 1999·Journal of Neuroscience Research·M Czapiga, C A Colton
Aug 27, 1999·Journal of Neuroscience Research·Y WangS Fedoroff
Mar 14, 2000·Journal of Neuroimmunology·M R WaltonM Dragunow
Jul 17, 2001·Microscopy Research and Technique·J D Glass, S L Wesselingh
Aug 31, 2001·Microscopy Research and Technique·C KaurE A Ling
Oct 2, 2001·Neurochemistry International·E N BenvenisteG M O'Keefe
Apr 18, 2002·Neurobiology of Aging·Valerie A M VincentGreer M Murphy
Jul 12, 2002·Journal of Neuroscience Research·Yong B LeeSeung U Kim
Jul 23, 2002·Neuroscience Letters·Christine Schermer, Christian Humpel
Jun 12, 2003·Journal of Neuroimmunology·Zhihua Qiu, Donna L Gruol
Aug 21, 2003·Neurobiology of Aging·Olivera M Mitrasinovic, Greer M Murphy

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alzheimer's Disease: Microglia

Microglia are a type of glial cell found throughout the brain and spinal cord. Microglia have been found to be associated with Alzheimer's disease development and progression. Here are the latest discoveries pertaining to Alzheimer's disease and microglia.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.