PMID: 11904070Mar 21, 2002Paper

Biologic characteristics of fibroblast cells cultured from the knee ligaments

Chinese Journal of Traumatology = Zhonghua Chuang Shang Za Zhi
Honghui ChenCancan Zhong

Abstract

To culture fibroblast cells from the knee ligaments and to study the biological characteristics of these cells. Cells of the anterior cruciate ligament (ACL) and the medial collateral ligament (MCL) from New Zealand white rabbit were cultured in vitro. Cellular growth and expression of the collagen were analyzed. Moreover, an in vitro wound closure model was established and the healing of the ACL and the MCL cells was compared. Maximal growth for all these cells were obtained with Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, but RPMI 1640 and Ham's F12 media were not suitable to maintain these cells. Morphology of both ACL and MCL cells from New Zealand white rabbit was alike in vitro, but the MCL cells grew faster than the ACL cells. Both cell types produced similar amount of collagen in culture, but the ratio of collage type I to type III produced by ACL cells was higher than that produced by MCL cells. Wound closure assay showed that at 36 hours after injury, cell-free zones created in the ACL cultures were occupied partially by the ACL cells; in contrast, the wounded zone in the MCL cultures was almost completely covered by the cells. Although the ACL cells and the MCL cells from New Zealand ...Continue Reading

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