Biologic properties and enucleation of red blood cells from human embryonic stem cells.

Blood
Shi-Jiang LuRobert Lanza

Abstract

Human erythropoiesis is a complex multistep process that involves the differentiation of early erythroid progenitors to mature erythrocytes. Here we show that it is feasible to differentiate and mature human embryonic stem cells (hESCs) into functional oxygen-carrying erythrocytes on a large scale (10(10)-10(11) cells/6-well plate hESCs). We also show for the first time that the oxygen equilibrium curves of the hESC-derived cells are comparable with normal red blood cells and respond to changes in pH and 2,3-diphosphoglyerate. Although these cells mainly expressed fetal and embryonic globins, they also possessed the capacity to express the adult beta-globin chain on further maturation in vitro. Polymerase chain reaction and globin chain specific immunofluorescent analysis showed that the cells increased expression of beta-globin (from 0% to > 16%) after in vitro culture. Importantly, the cells underwent multiple maturation events, including a progressive decrease in size, increase in glycophorin A expression, and chromatin and nuclear condensation. This process resulted in extrusion of the pycnotic nuclei in up to more than 60% of the cells generating red blood cells with a diameter of approximately 6 to 8 mum. The results show...Continue Reading

References

May 17, 1990·Nature·F YamamotoS Hakomori
Jun 1, 1967·The Journal of Cell Biology·E Skutelsky, D Danon
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·C PeschleP Comi
Sep 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·D S KaufmanJ A Thomson
May 1, 2002·Nature Biotechnology·Thi My Anh Neildez-NguyenLuc Douay
Feb 6, 2004·Blood Cells, Molecules & Diseases·G R HonigL Vida
Mar 5, 2004·The New England Journal of Medicine·Chad A CowanDouglas A Melton
Dec 28, 2004·Nature Biotechnology·Marie-Catherine GiarratanaLuc Douay
Feb 26, 2005·Experimental Hematology·George Stamatoyannopoulos
Aug 12, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sandra PilatHannes Klump
Oct 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Petter S WollDan S Kaufman
Dec 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yuan WangGeorge Q Daley
Jan 18, 2006·Stem Cells·Kristian M BowlesRoger A Pedersen
May 19, 2006·The Journal of Biological Chemistry·Shivani SoniManjit Hanspal
Jun 7, 2006·Current Opinion in Hematology·Paul A Ney
Dec 13, 2006·Experimental Hematology·Emmanuel N OlivierEric E Bouhassira
May 9, 2007·Nature Methods·Shi-Jiang LuRobert Lanza
Nov 22, 2007·Science·Junying YuJames A Thomson
Feb 12, 2008·Nature Cell Biology·Peng JiHarvey F Lodish
May 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Joan IsernMargaret H Baron

❮ Previous
Next ❯

Citations

Jun 24, 2011·Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·H BönigE Seifried
Jan 5, 2013·In Vitro Cellular & Developmental Biology. Animal·Abdolkhaleg Deezagi, Mahkameh Abedi-Tashi
Feb 20, 2010·International Journal of Hematology·Hiroshi SakamotoMinetaro Ogawa
Dec 25, 2010·International Journal of Hematology·Yukio NakamuraRyo Kurita
Jun 1, 2012·International Journal of Hematology·Padma Priya Togarrati, Kran Suknuntha
Jun 1, 2012·International Journal of Hematology·Yasuhiro EbiharaKohichiro Tsuji
Mar 12, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yiqi Seow, Matthew J Wood
Sep 5, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Xiaosong HuangLinzhao Cheng
Aug 25, 2009·Nature Methods·Tanya HatzistavrouAndrew G Elefanty
Oct 10, 2012·Proceedings of the National Academy of Sciences of the United States of America·Stella T ChouMitchell J Weiss
Aug 7, 2013·Proceedings of the National Academy of Sciences of the United States of America·Mi-Ok LeeHyuk-Jin Cha
Sep 10, 2010·Stem Cells and Development·Hera Andrade-ZaldívarAntonio De León-Rodríguez
Mar 26, 2011·Stem Cells and Development·Jessica DiasIgor I Slukvin
Sep 10, 2011·Tissue Engineering. Part a·Hyun Ok Kim, Eun Jung Baek
May 16, 2009·Current Opinion in Hematology·Anna Rita MigliaccioGiovanni Migliaccio
Apr 27, 2011·Current Opinion in Hematology·Christelle MazurierHélène Lapillonne
Apr 5, 2012·Cold Spring Harbor Perspectives in Medicine·Natasha Arora, George Q Daley
Aug 3, 2013·The Journal of Clinical Investigation·Shinji HirataKoji Eto
Jul 2, 2011·International Journal of Nanomedicine·N SuwanpayakP P Yupapin
Aug 9, 2011·International Journal of Nanomedicine·S MitathaP P Yupapin
Oct 8, 2011·International Journal of Nanomedicine·S MitathaP P Yupapin
Oct 5, 2010·Haematologica·Igor I Slukvin
May 25, 2011·World Journal of Stem Cells·Jean-Marie RamirezJohn De Vos
Jan 4, 2013·Stem Cells Translational Medicine·Eric E Bouhassira
Dec 18, 2013·Cellular & Molecular Immunology·Zhengping JiangXuetao Cao
May 12, 2010·Regenerative Medicine·Joanne C MountfordMarc L Turner
Mar 26, 2011·Stem Cells International·Erin A Kimbrel, Shi-Jiang Lu
Oct 19, 2011·Stem Cells International·Ganesan KeerthivasanJohn D Crispino
Jul 26, 2011·Stem Cells International·Takashi HiroyamaYukio Nakamura
Feb 7, 2012·Cell Stem Cell·Anna Rita MigliaccioMichel Sadelain

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Feng MaKohichiro Tsuji
Proceedings of the National Academy of Sciences of the United States of America
D S KaufmanJ A Thomson
© 2022 Meta ULC. All rights reserved