PMID: 15226594Jul 1, 2004Paper

Biological activities of tethered equine chorionic gonadotropin (eCG) and its deglycosylated mutants

The Journal of Reproduction and Development
K S MinKunio Shiota

Abstract

Equine chorionic gonadotropin (eCG), which consists of highly glycosylated alpha- and beta-subunits, is a unique member of the gonadotropin family because it elicits response characteristics of both follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in species other than the horse. In this study, recombinant tethered-eCG as well as its deglycosylated mutants were produced to determine if alpha- and beta- subunits can be synthesized as a single polypeptide chain (tethered-eCG) and display biological activity. We found that tethered-eCG (T- betaalpha) had both LH- and FSH-like activities comparable to dimeric eCG. Luteinizing hormone-like activity of tethered-eCGs deglycosylated at Asn(56) (T-betaalpha56) was decreased. In contrast, LH-like activity of eCG without O-glycosylated carboxyl-terminal peptide (CTP) (T-betacalpha) was slightly decreased but still similar to T-betaalpha. Double mutation at Asn(56) and CTP (T-betacalpha56) caused marked decrease in the activity, indicating that both glycosylations at Asn(56) and CTP are involved in LH-like activity in the tethered form. Interestingly, FSH-like activity remained in all deglycosylated eCG mutants (T-betaalpha56, T-betacalpha and T-betacalpha56) as well as T-be...Continue Reading

References

Jan 25, 1978·Biochimica Et Biophysica Acta·H PapkoffJ Ramachandran
Jan 1, 1981·Annual Review of Biochemistry·J G Pierce, T F Parsons
Jan 6, 1995·Science·J L PomerantzC O Pabo
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·T SugaharaI Boime

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Citations

Jan 31, 2012·Reproductive Biology and Endocrinology : RB&E·Seong-Jo YunKwan-Sik Min
Jan 27, 2012·Animal Reproduction Science·P J NaidansurenK S Min
Sep 13, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Purevjargal NaidansurenKwan-Sik Min
Aug 10, 2017·Balsaeng'gwa saengsig·Jong-Ju ParkKwan-Sik Min

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