Biological activity of harpin produced by Pantoea stewartii subsp. stewartii

Molecular Plant-microbe Interactions : MPMI
M AhmadD L Coplin

Abstract

Pantoea stewartii subsp. stewartii causes Stewart's wilt of sweet corn. A hypersensitive response and pathogenicity (Hrp) secretion system is needed to produce water-soaking and wilting symptoms in corn and to cause a hypersensitive response (HR) in tobacco. Sequencing of the hrp cluster revealed a putative harpin gene, hrpN. The product of this gene was overexpressed in Escherichia coli and shown to elicit the HR in tobacco and systemic resistance in radishes. The protein was designated HrpN(Pnss). Like other harpins, it was heat stable and protease sensitive, although it was three- to fourfold less active biologically than Erwinia amylovora harpin. We used antibodies to purified HrpN(Pnss) to verify that hrpN mutants could not produce harpin. This protein was secreted into the culture supernatant and was produced by strains of P. stewartii subsp. indologenes. In order to determine the importance of HrpN(Pnss) in pathogenesis on sweet corn, three hrpN::Tn5 mutants were compared with the wild-type strain with 50% effective dose, disease severity, response time, and growth rate in planta as parameters. In all tests, HrpN(Pnss) was not required for infection, growth, or virulence in corn or endophytic growth in related grasses.

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Citations

Jul 21, 2011·Molecular Plant-microbe Interactions : MPMI·Dong-Hai PengMing Sun
Mar 7, 2006·Applied and Environmental Microbiology·Anne-Marie GrenierYvan Rahbé
May 6, 2003·Journal of Bacteriology·Jung-Gun KimIngyu Hwang
Mar 4, 2006·Journal of Bacteriology·Mee-Ngan YapAmy O Charkowski
Jul 31, 2004·Annual Review of Phytopathology·James R Alfano, Alan Collmer
Oct 26, 2013·Molecular Plant-microbe Interactions : MPMI·E VergneE Chevreau
Oct 31, 2012·Research in Microbiology·William H DePas, Matthew R Chapman
Mar 22, 2008·FEMS Microbiology Ecology·Thimmaraju RudrappaHarsh P Bais
Feb 23, 2007·The Journal of Biological Chemistry·Jonghee OhIngyu Hwang
Oct 4, 2020·International Journal of Molecular Sciences·Anastasiia O KosolapovaAnton A Nizhnikov

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