PMID: 9434274Jan 22, 1998Paper

Biological activity of lysozyme after entrapment in poly(d,l-lactide-co-glycolide)-microspheres

Pharmaceutical Research
R Ghaderi, J Carlfors

Abstract

The purpose of this study was to investigate the process of preparing microspheres for maximising entrapment efficiently (EE) and retained biological activity (RBA) of peptides and proteins. A controlled-release formulation based on poly(d,l-lactide-co-glycolide) was designed and produced using a small-scale double emulsion method. These PLG microspheres contained a model peptide, lysozyme. The retained bioactivity of the incorporated lysozyme was determined by bacterial assay. The size distributions and the morphology of the microspheres were characterized. The RBA and EE improved when the PLG concentration in the organic phase of the emulsion was increased. A high lysozyme concentration in the inner water phase of the emulsion resulted in decreased EE and an increase in the proportion of fragmented particles. The RBA of lysozyme in the microspheres varied between 30 and 80% with changes to the process. The study shows that the RBA of lysozyme in PLG microspheres is strongly dependent on the experimental conditions for preparing the microspheres. Measurement of the EE alone, without the RBA is insufficient to evaluate the efficacy of the designed delivery system.

Citations

Mar 18, 2006·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Hongliang JiangKangjie Zhu
May 30, 2015·Pharmacological Reviews·Miia KovalainenKarl-Heinz Herzig
May 9, 2001·The Journal of Pharmacy and Pharmacology·Y H LiaoG P Martin
Sep 27, 2012·Angewandte Chemie·Samuel E ReinholdSteven P Schwendeman
Jul 17, 2013·Drug Development and Industrial Pharmacy·Hesham M TawfeekGillian A Hutcheon
Aug 30, 2008·Critical Reviews in Food Science and Nutrition·Christina KriegelJochen Weiss
Oct 29, 2015·BioMed Research International·Inmaculada Ortega-OllerJose Manuel Peula-García
Mar 15, 2018·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Parker W Lee, Jonathan K Pokorski
Jan 15, 2005·Pharmaceutical Research·Anne Aubert-PouësselJean-Pierre Benoît
Mar 2, 2021·International Journal of Pharmaceutics·Kurt D RistrophRobert K Prud'homme
Dec 10, 1999·Journal of Pharmaceutical Sciences·H Sah
May 1, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Marc SutterWim Jiskoot
Nov 21, 2007·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Cuifang CaiThomas Kissel
Apr 5, 2007·Biomagnetic Research and Technology·Hong ZhaoUrs O Häfeli
Jun 6, 2009·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Yiwei WangAllan G A Coombes

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