PMID: 2484952Jan 1, 1989Paper

Biological and binding studies of acidic fibroblast growth factor in the presence of substituted dextran

Journal of Biomaterials Science. Polymer Edition
M TardieuD Barritault

Abstract

Heparin has been shown to interact with acidic fibroblast growth factor (aFGF) and to potentiate the biological activity of aFGF on fibroblastic cells. Water-soluble dextran substituted with methyl carboxylic benzylamine and sulfonate groups has been shown to mimic the effect of heparin in its anticoagulant and anticomplement activity. We have studied the effect of a dextran derivative named E (DDE), which had an anticoagulant activity equivalent to 0.5 IU heparin/mg, on the mitogenic activity of aFGF on Chinese hamster fibroblasts (CCL39). DDE interacts with aFGF in a comparable manner to heparin. We have shown that 20 micrograms of heparin or 400 micrograms of DDE added to 1 ml of culture medium has no effect on cell proliferation alone but potentiates the mitogenic activity of aFGF ten fold if aFGF is added at doses corresponding to half maximum stimulation (ED50). We have also studied the effect of various concentrations of heparin and DDE on the binding of 125I-aFGF on bovine brain membranes. Interestingly, the binding of 125I-aFGF increased three-fold as the concentration of heparin was increased up to 0.2 microgram/ml. At 1 microgram/ml of heparin, the amount of bound 125I-aFGF is comparable to that obtained in the absen...Continue Reading

References

Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A B SchreiberT Maciag
Apr 15, 1988·Biochemical and Biophysical Research Communications·T K RosengartT Maciag
Jan 1, 1985·Biomaterials·M MauzacM D Kazatchkine
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M MoennerD Barritault
Apr 15, 1988·Biochemical and Biophysical Research Communications·A GotoT Sugimoto
Sep 1, 1986·Journal of Cellular Physiology·D Gospodarowicz, J Cheng
Apr 14, 1986·Biochemical and Biophysical Research Communications·J CourtyD Barritault
Jun 30, 1986·Biochemical and Biophysical Research Communications·S UhlrichY Courtois
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M Klagsbrun, Y Shing

❮ Previous
Next ❯

Citations

Jan 1, 1992·Progress in Growth Factor Research·D LedouxD Barritault
Dec 1, 1995·Bone·F BlanquaertJ P Caruelle
Jan 1, 1993·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·D LetourneurJ Jozefonvicz
Oct 1, 1994·Pathology, Research and Practice·A MeddahiD Barritault
Oct 16, 2016·Biomacromolecules·Samantha J PaluckHeather D Maynard
Jul 9, 1999·Journal of Biomedical Materials Research·F BlanquaertJ P Caruelle
Feb 13, 2003·Journal of Biomedical Materials Research. Part a·Frédéric BlanquaertMichèle Tardieu
Jan 1, 1991·Journal of Biomaterials Science. Polymer Edition·T Avramoglou, J Jozefonvicz
Jan 1, 1990·Journal of Biomaterials Science. Polymer Edition·J Jozefonvicz, M Jozefowicz
Jun 1, 1993·Journal of Biomedical Materials Research·N OturanM Jozefowicz
Apr 1, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Q EscartinJ L Saffar
Jun 5, 2020·Biomacromolecules·Chanel C LaJayachandran N Kizhakkedathu
Feb 1, 1996·International Journal of Biological Macromolecules·A MeddahiW Hornebeck

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Biomaterials Science. Polymer Edition
S BerradaM F Harmand
In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association
D LetourneurJ Jozefonvicz
American Journal of Respiratory Cell and Molecular Biology
J J Castellot
© 2022 Meta ULC. All rights reserved