PMID: 611155Jan 1, 1977Paper

Biological and biochemical characterization of a latent subacute sclerosing panencephalitis (SSPE) virus infection in tissue culture

Journal of Medical Virology
V KratzschV ter Meulen

Abstract

The present investigation describes the biological and biochemical properties of a persistent SSPE virus infection. Persistently infected cells were derived by cocultivation of infected brain cells and uninfected Vero cells, and cultures were maintained by normal subculturing methods. No infectious virus was ever released from these cultures, and all attempts to induce infectious virus release were unsuccessful. Biological assays showed that infected cells contained nucleocapsid and salt-dependent hemagglutinin antigens, whereas the normal hemagglutinin appeared not to be present. Electron microscopic examination demonstrated the presence of both intranuclear and cytoplasmic nucleocapsids together with the release of virus particles (defective?) from the cell membrane. Biochemical analysis demonstrated that approximately 90% of the intracellular genomic RNA was defective or subgenomic although a small quantity of infectious genomes was present. It is proposed that the large quantities of defective genomes in the infected cells are the major factor in the maintenance of this persistent infection.

References

Oct 1, 1976·The Journal of General Virology·P V ShirodariaE A Gould
Jun 1, 1976·Medical Microbiology and Immunology·R K Rima, S J Martin
Oct 1, 1973·Journal of Virology·M V HaspelF Rapp
Jan 1, 1974·Medical Microbiology and Immunology·W W HallE Gould
Jan 1, 1967·Archiv für die gesamte Virusforschung·E Norrby
Oct 1, 1972·Japanese Journal of Medical Science & Biology·Y DoiT Koto
Jan 1, 1974·Medical Microbiology and Immunology·M P Kiley, F E Payne
Jan 1, 1974·Intervirology·P SethJ L Melnick
Oct 1, 1969·Proceedings of the Society for Experimental Biology and Medicine·L Horta-BarbosaJ L Sever

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Citations

Jan 1, 1981·Archives of Virology·F KobuneA Shishido
Jan 1, 1982·Archives of Virology·R VainionpääP Arstila
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P Münzel, K Koschel
Dec 1, 1980·Japanese Journal of Medical Science & Biology·I Prasad, A Mnere
Jan 1, 1982·Archives of Virology·J R Stephenson, V ter Meulen
Apr 27, 2004·Microbiology and Immunology·Hiroshi IshidaHisashi Ogura

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