Biological and growth properties of a 20-methyl-cholanthrene transformed murine fibroblast cell line (PMM-14) established from primary culture

Cancer Letters
P MukherjeeS K Das

Abstract

Some major transformation related biological and growth properties have been analysed in an in vitro, system to characterize the PMM-14 cell line, a 20-methylcholanthrene induced transformed mouse embryonic fibroblast cell line. The population doubling time of this cell line was 19 h with moderately high saturation density and plating efficiency. Attachment detachment properties showed reduced adhesion to substratum. Cytogenetic study revealed the existence of a large number of Robertsonian biarmed chromosomes with hypertriploid modal range (60-69) represented by about 21% cells. These cells showed enhanced concanavalin A agglutinability which was reduced by trypsin treatment. Its neoplastic nature was finally established by its ability to grow in agar with a high plating efficiency.

References

Nov 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·S I ShinR Pollack
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J C Barrett, P O Ts'o
Mar 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·M M Burger
Aug 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·M Inbar, L Sachs
Nov 20, 1973·Biochemistry·M C GlickL Sachs
Mar 12, 1980·Biochimica Et Biophysica Acta·L A Smets

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