Biological and molecular characterization of tospoviruses in Thailand

Archives of Virology
P ChiemsombatPreyapan Pongsapich

Abstract

Twenty-eight isolates of tospoviruses associated with tomato, pepper, cucurbits, peanut, and Physalis plants collected from fields in different regions of Thailand were characterized. On the basis of N gene and protein sequence relationships, three tospoviruses were identified, namely Watermelon silver mottle virus (WSMoV), Capsicum chlorosis virus (CaCV), and Melon yellow spot virus (MYSV). CLUSTAL analysis of selected N protein sequences showed different isolates of CaCV in three distinct clades. Based on necrosis symptoms on tomato and their 93% identity to CaCV isolates in the other two clades, CaCV-TD8, CaCV-AIT and CaCV-KS16-Thailand tomato tospovirus were designated as CaCV-tomato necrosis strain. A phylogenetic tree based on the 413-amino-acid Gc fragment of the CaCV-Pkk isolate supported the existence of three distinct CaCV clades. Vigna unguiculata produced concentric rings useful for discriminating the Thai CaCV peanut isolates from tomato or pepper isolates. By using reverse transcription polymerase chain reaction with species-specific primers, the three tospoviruses could be detected in mixed infections in watermelon and Physalis, as well as in the bodies of thrips vectors, Thrips palmi and Scirtothrips dorsalis, c...Continue Reading

References

Feb 1, 1993·The Journal of General Virology·A C de AvilaD Peters
Jul 21, 2004·Briefings in Bioinformatics·Sudhir KumarMasatoshi Nei
Aug 5, 2005·Annual Review of Phytopathology·Anna E WhitfieldThomas L German
Jan 26, 2006·Journal of Inherited Metabolic Disease·M Di RoccoJ Jaeken
May 1, 2000·Molecular Plant Pathology·S Adkins

❮ Previous
Next ❯

Citations

Nov 26, 2010·Archives of Virology·Channarong SeepibanSupat Attathom
May 3, 2013·PloS One·Ratthaphol CharlermrojChristopher T Elliott
Jan 1, 2014·PloS One·Numrin ThaitrongNitsara Karoonuthaisiri
Sep 4, 2014·Environmental Entomology·Youngjin ParkYonggyun Kim
Jul 5, 2015·Journal of Virological Methods·Channarong SeepibanOraprapai Gajanandana
Mar 20, 2014·Journal of Virological Methods·Saengsoon CharoenvilaisiriOraprapai Gajanandana
Apr 22, 2015·PloS One·Aaron M DickeyCindy L McKenzie
Feb 9, 2011·Phytopathology·Suresh R KunkalikarKankanallu S Ravi
Jun 20, 2014·Analytical Chemistry·Ratthaphol CharlermrojNitsara Karoonuthaisiri
Mar 30, 2017·Archives of Virology·S CheewachaiwitO Chatchawankanphanich

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.