Biological and MRI characterization of biomimetic ECM scaffolds for cartilage tissue regeneration

Biomaterials
Sriram RavindranAnne George

Abstract

Osteoarthritis is the most common joint disorder affecting millions of people. Most scaffolds developed for cartilage regeneration fail due to vascularization and matrix mineralization. In this study we present a chondrogenic extracellular matrix (ECM) incorporated collagen/chitosan scaffold (chondrogenic ECM scaffold) for potential use in cartilage regenerative therapy. Biochemical characterization showed that these scaffolds possess key pro-chondrogenic ECM components and growth factors. MRI characterization showed that the scaffolds possess mechanical properties and diffusion characteristics important for cartilage tissue regeneration. In vivo implantation of the chondrogenic ECM scaffolds with bone marrow derived mesenchymal stem cells (MSCs) triggered chondrogenic differentiation of the MSCs without the need for external stimulus. Finally, results from in vivo MRI experiments indicate that the chondrogenic ECM scaffolds are stable and possess MR properties on par with native cartilage. Based on our results, we envision that such ECM incorporated scaffolds have great potential in cartilage regenerative therapy. Additionally, our validation of MR parameters with histology and biochemical analysis indicates the ability of MRI...Continue Reading

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Citations

Aug 29, 2017·Tissue Engineering. Part C, Methods·Mrignayani KotechaHoward Halpern
Jun 8, 2019·Molecular Medicine Reports·Hui XingTao Jiang
Aug 5, 2017·Materials·Marina Trevelin SouzaAldo R Boccaccini
Mar 3, 2017·Journal of Materials Science. Materials in Medicine·Wei ZhuXisheng Weng
Jun 7, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Nandana BhardwajBiman B Mandal
Nov 12, 2015·Frontiers in Physiology·Sriram RavindranRaghuvaran Narayanan
Jun 2, 2017·TheScientificWorldJournal·José Carlos Viana RibeiroVilma Lima
Jun 12, 2018·Frontiers in Physiology·Chun-Chieh HuangSriram Ravindran
Jan 28, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jun LiuXingdong Zhang
Dec 29, 2020·Frontiers in Bioengineering and Biotechnology·Yanhong ZhaoYanmei Zhao
Jun 8, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Piyali DasSamit Kumar Nandi
Jul 10, 2017·ACS Biomaterials Science & Engineering·Anna D DikinaEben Alsberg
Sep 24, 2020·ACS Applied Materials & Interfaces·Liqun NingVahid Serpooshan

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