PMID: 9638276Jun 25, 1998Paper

Biological characterization and genetic diversity of Mexican isolates of Trypanosoma cruzi

Acta Tropica
V López-OlmosB Espinoza

Abstract

The present work reports the in vitro biological characterization of 17 Trypanosoma cruzi isolates from southern and central México, and compares these results to those of four South American strains and one clone from Brazil. The parameters evaluated were growth rates, percentage of parasites undergoing transformation from epimastigotes to trypomastigotes, infectivity to, and in vitro killing of cultured Vero and P388 cells. Isoenzyme patterns of 11 enzymatic systems and 16 loci were also determined for the Mexican isolates. The parasites showed differences in growth, depending if they were cultured in LIT with hemin or in Grace's media. Transformation was obtained only in Grace's medium and differences were observed between the stocks. Stocks Z10 and Z21 showed the highest percentage of transformation within the Mexican isolates (39 and 41%, respectively). A second group showed percentages of transformation between 15 and 28%. In contrast, the South American strains showed higher rates of transformation (36-65%). Infection of cultured cells by isolates Z10 and H5 was evaluated in both Vero and P388 cells. Differences were observed both in the percentage of infected cells as well as in the number of amastigotes per cell. Diffe...Continue Reading

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Citations

Jan 31, 2012·Journal of Biomedicine & Biotechnology·Deyanira Pérez-MoralesBertha Espinoza
Feb 5, 2002·Journal of Clinical Microbiology·Marie-France BossenoSimone Frédérique Brenière
Jul 15, 2009·Experimental Parasitology·Deyanira Pérez-MoralesBertha Espinoza
Aug 22, 2009·Acta Tropica·Nancy R Sturm, David A Campbell
Apr 4, 2001·Memórias do Instituto Oswaldo Cruz·R A Martínez-DíazA Gómez-Barrio
Nov 7, 2001·The Journal of Parasitology·R HernándezB Espinoza
Oct 3, 2020·Parasitology Research·Ruben D Arroyo-OlarteBertha Espinoza

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