PMID: 9188581Jul 1, 1997Paper

Biological characterization of human immunodeficiency virus type 1 clones derived from different organs of an AIDS patient by long-range PCR

Journal of Virology
M T DittmarR A Weiss

Abstract

In order to characterize the biological properties of human immunodeficiency virus type 1 (HIV-1) variants from different tissues (peripheral blood mononuclear cells [PBMC], lymph node, spleen, brain, and lung) of one patient, we have chosen long-range PCR to amplify virtually full-length HIV proviruses and to construct replication-competent viruses by adding a patient-specific 5' long terminal repeat. To avoid selection during propagation in CD4+ target cells, we transfected 293 cells and used the supernatants from these cells as challenge viruses for tropism studies after titration on human PBMC. Despite differences in the V3 loop of the major variants found in brain and lung compared to lymphoid tissues all recombinant HIV clones obtained showed identical cell tropism and replicative kinetics. After infection of human PBMC these viruses replicated with similar kinetics, with a slow/low-titer, non-syncytium-inducing phenotype. In contrast to the prediction of macrophage tropism, drawn from the V3 loop sequence, none of these viruses infected monocyte-derived macrophages. The challenge of blood dendritic cells by these recombinant viruses in the presence of tumor necrosis factor alpha, granulocyte-macrophage colony-stimulating...Continue Reading

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Citations

Jun 5, 1999·AIDS Research and Human Retroviruses·M T DittmarL Gürtler
Sep 10, 2003·Journal of Leukocyte Biology·Maureen M GoodenowJohn W Sleasman
Nov 15, 2012·AIDS Research and Human Retroviruses·Shivaji K JadhavAtmaram H Bandivdekar
Apr 3, 2001·AIDS Research and Human Retroviruses·I N KoulinskaB Renjifo
Aug 13, 1999·AIDS Research and Human Retroviruses·S HibbittsP R Clapham
Mar 4, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S E HowieD J Harrison
Nov 28, 2007·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Jeffrey S Barrett
Mar 18, 2011·Journal of Medical Virology·Shivaji K JadhavAtmaram H Bandivdekar
Jul 13, 2000·AIDS Research and Human Retroviruses·V A NovitskyM Essex
Nov 21, 2001·British Medical Bulletin·P R Clapham, A McKnight
Mar 21, 1998·Current Opinion in Biotechnology·G R Taylor, P Robinson
Jan 22, 2000·Journal of Virological Methods·L HoltermanJ Heeney

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