Biological characterization of Trypanosoma cruzi zymodemes: in vitro differentiation of epimastigotes and infectivity of culture metacyclic trypomastigotes to mice

Experimental Parasitology
G SanchezA Solari

Abstract

Thirty-one Trypanosoma cruzi isolates from Chile, Peru, and Bolivia were studied in their capacity to differentiate in vitro from epimastigotes to metacyclic trypomastigotes on TAU-3AAG medium. Zymodeme 1 parasites displayed the best level of differentiation, which ranges from 60 to 90% depending on the isolate. Zymodeme 2 parasites exhibited highly heterogenous differentiation rates. This differentiation method permits the obtention of large amounts of metacyclic trypomastigotes from zymodeme 1 parasites. Metacyclic trypomastigotes obtained in vitro were infective to nude Balb/c hybrid mice. Zymodeme 1 parasites produced high parasitemias in this murine model; in contrast, zymodeme 2 parasites displayed lower parasitemias. Of a total of 27 T. cruzi isolates, 20 proved to be infective to mice, 12 gave enough parasites for further studies, and 8 of these were used for biological characterization. Results are compared with the infective clone Dm28 and Tulahuén strains maintained since 1954 in mice.

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Citations

May 1, 1993·Acta Tropica·P J Myler
May 5, 2010·The American Journal of Tropical Medicine and Hygiene·Daniel AdesseDavid C Spray
Jul 24, 1998·Experimental Parasitology·A SolariG Sanchez
May 1, 1997·Revista Da Sociedade Brasileira De Medicina Tropical·L Lauria-PiresA R Teixeira
Feb 26, 2013·PLoS Neglected Tropical Diseases·Wuelton Marcelo MonteiroMaria das Graças Vale Barbosa

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