Biological effects and comparative cytotoxicity of thermal transformed asbestos-containing materials in a human alveolar epithelial cell line

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Federica GiantomassiArmanda Pugnaloni

Abstract

Asbestos fibres can be transformed into potentially non-hazardous silicates by high-temperature treatment via complete solid-state transformation. A549 cells were exposed to standard concentrations of raw cement asbestos (RCA), chrysotile and cement asbestos subjected to an industrial process at 1200 degrees C (Cry_1200 and KRY.AS, respectively), raw commercial grey cement (GC). Cell growth rate and viability (MTT test) were detected in vitro. RCA and KRY.AS subjected to comprehensive microstructural study by electron microscopy were further in vitro assayed to compare their cytotoxic potential by morphostructural studies, proliferation index (Ki-67 antigen), apoptosis induction (AO/EB staining) assays and detection of intracellular reactive oxygen species (ROS) with the fluorescent DCFA dye. More severe cytotoxic damage was induced by RCA than by KRY.AS after each incubation period. Exposure to KRY.AS and GC resulted in comparable cell growth rates and cytotoxic effects. Cells incubated with RCA showed greater apoptotic induction and ROS production and a lower cell proliferation index than those exposed to KRY.AS. Chrysotile asbestos and RCA subjected to heat treatment underwent complete microstructure transformation. The fina...Continue Reading

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Citations

Nov 8, 2011·Analytical and Bioanalytical Chemistry·Alessandro PacellaArmanda Pugnaloni
Jul 13, 2013·Analytical and Bioanalytical Chemistry·Seydou YaoCyril Petibois
Feb 6, 2013·Journal of Hazardous Materials·Alberto VianiGiancarlo Urso
Jan 20, 2012·Journal of Hazardous Materials·Alessandro F GualtieriGigliola Lusvardi
Nov 22, 2011·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Qingyu XiangYuan Huang
Mar 7, 2015·Toxicological Sciences : an Official Journal of the Society of Toxicology·Annalisa BernareggiGiuliano Zabucchi
Dec 4, 2014·Acta histochemica·Armanda PugnaloniAlessandro F Gualtieri
Mar 16, 2019·Critical Reviews in Toxicology·Craig A Poland, Rodger Duffin

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