Biological effects of anti-CD34-coated ePTFE vascular grafts. Early in vivo experimental results

Kardiochirurgia i torakochirurgia polska = Polish journal of cardio-thoracic surgery
Wojciech MrówczyńskiB H Walpoth

Abstract

To assess the biological activity of anti-CD34 antibody-coated ePTFE vascular prostheses. Indium(111)-labeled autologous thrombocytes were administered to 5 anesthetized pigs after the placement of femoral arterial and venous catheters. An arterio-venous fistula, created by the random interposition of 4 different ePTFE grafts (A = dry control, B = dry anti-CD34, C = wet control, D = wet anti-CD34), was blood perfused for 0, 10, 30, 60 and 120 minutes. Radioactivity of each graft was measured and expressed in cpm/mg. Morphological studies were performed to assess intraluminal deposition. The median radioactivity of graft B was significantly higher than that of graft A after 60 min (1074 vs. 18; p = 0.021) and 120 min (1990 vs. 25; p = 0.043) of perfusion. Similarly, graft D was significantly more active than graft C (60 min: 1388 vs. 26; p = 0.021 and 120 min: 2780 vs. 23; p = 0.021). Histological and SEM results confirmed the radio-labeling in-vivo studies by showing significantly more protein/cell and platelet depositions (p = 0.012). Anti-CD34-coated ePTFE grafts bound significantly more platelets/cells and proteins than their uncoated counterparts, confirming the bioactivity of the antibody. This process is time-dependent an...Continue Reading

Citations

May 4, 2017·Journal of Biomedical Materials Research. Part a·Sujan LamichhanePatrick Kelly
Oct 28, 2019·Journal of Biomedical Materials Research. Part a·Haoyong YuanZhongshi Wu
Oct 10, 2020·Frontiers in Cardiovascular Medicine·Peter ZillaTim Pennel
Feb 27, 2021·Respiratory Research·Gurpreet K AulakhBaljit Singh

❮ Previous
Next ❯

Methods Mentioned

BETA
scanning electron microscopy

Software Mentioned

ImageJ

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.