Biological effects of FoxJ2 over-expression.

Transgenic Research
F Martín-de-LaraJ Rey-Campos

Abstract

As reported previously, we have extensively studied FoxJ2, a member of the Fork Head transcription factors family. While the biochemical and functional structures of this transcription factor are well understood, its biological function remains unknown. Here, we present data that address this point using transgenic mouse technology. We found that the birth rate and the number of transgenic animals obtained when transferring embryos over-expressing the FoxJ2 protein were lower than those obtained with embryos over-expressing a control protein, suggesting FoxJ2 overexpression has a negative effect on embryonic development. Transient FoxJ2 transgenesis experiments have confirmed that FoxJ2 over-expression has a lethal effect on embryonic development from E10.5. Moreover, in vitro culture of FoxJ2-microinjected embryos demonstrated a significant developmental blockage, indicating that FoxJ2 could also have an effect on pre-implantation stages. Most probably, these negative effects of FoxJ2 over-expression during development also explain the low percentage of adult transgenic mice obtained. Furthermore, most of the transgenic mice that lived to adulthood did not show transgene expression. In fact, the only two adult transgenic anima...Continue Reading

References

Jan 1, 1995·Life Sciences·R AlbaneseA Wieraszko
Mar 24, 1995·Science·A G ReaumeJ Rossant
Aug 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M W McBurneyL Pickavance
Jun 1, 1996·Mechanisms of Development·E Kaufmann, W Knöchel
Feb 21, 1998·Genes & Development·E Y ShimK S Zaret
Mar 5, 1998·Circulation Research·J YaW H Lamers
Apr 20, 1999·Biology of Reproduction·S C JunejaG M Kidder
Apr 25, 2000·The Journal of Biological Chemistry·C Pérez-SánchezJ Rey-Campos
Sep 1, 2000·Journal of Molecular Biology·C Pérez-SánchezJ Rey-Campos
Aug 22, 2001·Biology of Reproduction·W A RoscoeG M Kidder
May 17, 2003·Toxicology Letters·Nils Gunnar Ilbäck, Torbjörn Stålhandske
Jun 6, 2003·Journal of Molecular Biology·María Ana Gómez-Ferrería, Javier Rey-Campos
Feb 8, 2006·Gene Expression Patterns : GEP·Vivian M ChoiMustafa K Khokha

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Citations

Mar 30, 2011·Human Reproduction Update·Maurizio ZuccottiSilvia Garagna
Mar 24, 2012·Journal of Cellular Biochemistry·Yingying WangChun Cheng
Feb 8, 2014·American Journal of Medical Genetics. Part a·Richard K TiltonKosuke Izumi
Feb 11, 2015·Pathology, Research and Practice·Xiaojun QiuYe Tian
May 6, 2014·Journal of Molecular and Cellular Cardiology·Bin-Bin ChengZong-Lai Jiang
May 18, 2016·Scientific Reports·Graham D JohnsonStephen A Krawetz
Oct 21, 2014·PLoS Genetics·Stefano ComazzettoDónal O'Carroll
Jan 16, 2013·PloS One·Gianpaolo TibollaMarco Falasca
Aug 26, 2010·British Journal of Pharmacology·Gabor Tigyi
Jul 1, 2020·Investigative Ophthalmology & Visual Science·Sasha M Milbeck, Sanjoy K Bhattacharya
Feb 6, 2010·Reproductive Biomedicine Online·Maurizio ZuccottiSilvia Garagna

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