Biological effects of low-dose-rate irradiation of pancreatic carcinoma cells in vitro using 125I seeds

World Journal of Gastroenterology : WJG
Zhong-Min WangAi-Wu Mao

Abstract

To determine the mechanism of the radiation-induced biological effects of 125I seeds on pancreatic carcinoma cells in vitro. SW1990 and PANC-1 pancreatic cancer cell lines were cultured in DMEM in a suitable environment. Gray's model of iodine-125 (125I) seed irradiation was used. In vitro, exponential phase SW1990, and PANC-1 cells were exposed to 0, 2, 4, 6, and 8 Gy using 125I radioactive seeds, with an initial dose rate of 12.13 cGy/h. A clonogenic survival experiment was performed to observe the ability of the cells to maintain their clonogenic capacity and to form colonies. Cell-cycle and apoptosis analyses were conducted to detect the apoptosis percentage in the SW1990 and PANC-1 cells. DNA synthesis was measured via a tritiated thymidine (3H-TdR) incorporation experiment. After continuous low-dose-rate irradiation with 125I radioactive seeds, the survival fractions at 2 Gy (SF2), percentage apoptosis, and cell cycle phases of the SW1990 and PANC-1 pancreatic cancer cell lines were calculated and compared. The survival fractions of the PANC-1 and SW1990 cells irradiated with 125I seeds decreased exponentially as the dose increased. No significant difference in SF2 was observed between SW1990 and PANC-1 cells (0.766±0.063...Continue Reading

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Citations

Sep 30, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yi-Liang BiYan Liu
Jun 14, 2017·International Journal of Radiation Biology·Jony M GeraldoLídia M Andrade
Jan 25, 2020·International Journal of Radiation Biology·Sheng-Nan JiaZe-Cheng Yang
Jan 29, 2019·Biological & Pharmaceutical Bulletin·Zhiwei GuDekang Gao

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