Biological fate of sulphur mustard: identification of valine and histidine adducts in haemoglobin from casualties of sulphur mustard poisoning

Xenobiotica; the Fate of Foreign Compounds in Biological Systems
R M BlackR W Read

Abstract

1. Analytical methods were developed for the detection of N-terminal valine and histidine adducts in haemoglobin alkylated with sulphur mustard. 2. N-(2-hydroxyethylthioethyl)-N-terminal valine was selectively cleaved from globin with the Edman reagent pentafluorophenyl isothiocyanate. The resulting thiohydantoin derivative was analysed by high resolution gc-ms using negative ion chemical ionization. An alternative procedure, involving acid hydrolysis of globin to its constituent amino acids and conversion of the adduct to its di-TBDMS derivative, was less sensitive. 3. N-(2-hydroxyethylthioethyl)histidine was analysed, after acid hydrolysis of globin, as its fluorenylmethyloxycarbonyl derivative by lc-ms-ms using electrospray ionisation and selected reaction monitoring. 4. N-(2-hydroxyethylthioethyl)valine and (2-hydroxyethylthioethyl)histidine were detected in globin isolated from a rat treated percutaneously with sulphur mustard, and in globin from five blood samples collected from human casualties of sulphur mustard poisoning. The adducts are proposed as biological markers of sulphur mustard poisoning. in addition to urinary metabolites and DNA adducts.

References

Feb 1, 1995·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·R M Black, R W Read
Jan 1, 1994·Methods in Enzymology·S R Tannenbaum, P L Skipper

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Citations

Jul 19, 2013·Chemical Research in Toxicology·Vanessa R Thompson, Anthony P DeCaprio
Dec 5, 2015·Inhalation Toxicology·Mohammad Reza KhazdairVahideh Ghorani
Sep 14, 2015·Journal of Analytical Toxicology·Jin Young Lee, Yong Han Lee
Jul 28, 2011·The Ocular Surface·Alireza Baradaran-RafiiScheffer C G Tseng
Mar 23, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mia HalmePaula Vanninen
Dec 19, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Robin M Black
May 25, 2005·Fundamental & Clinical Pharmacology·Mahdi Balali-Mood, Mehrdad Hefazi
Jul 31, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Amanda S Appel, Brian A Logue
Aug 11, 2004·European Journal of Mass Spectrometry·Vladimir G Zaikin, John M Halket
Nov 24, 2018·Drug Testing and Analysis·Markus SiegertHarald John
Sep 14, 2019·Archives of Toxicology·Robin LülingDirk Steinritz

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