PMID: 7543584Sep 1, 1995Paper

Biological function of the low-pH, fusion-inactive conformation of rabies virus glycoprotein (G): G is transported in a fusion-inactive state-like conformation

Journal of Virology
Y GaudinR Ruigrok

Abstract

The glycoprotein (G) of rabies virus can assume at least three different conformations: the native (N) state detected at the viral surface above pH 7; the activated (A) hydrophobic state, which is probably involved in the first steps of the fusion process; and the fusion-inactive (I) conformation. There is a pH-dependent equilibrium between these states, the equilibrium being shifted towards the I state at low pH. It has been supposed that the transition from the N to the I state mediates membrane fusion. By using a lipid-mixing assay, we studied the kinetics of fusion and fusion inactivation for two rabies virus strains, PV and CVS. In addition, by using electron microscopy and a trypsin sensitivity assay, we analyzed the kinetics of the conformational change towards the I state for both strains. Although the PV strain fuses faster, inactivation and the conformational change of PV G occur more slowly than for the CVS strain. This suggests that the structural transition towards the I state is irrelevant to the fusion process. Immunofluorescence and immunoprecipitation experiments performed with infected cells and two different monoclonal antibodies, one specific for the N form of G and one which recognizes both the N and the I ...Continue Reading

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Citations

May 20, 1999·Molecular Membrane Biology·Y GaudinR Ruigrok
Aug 10, 2000·The Journal of Cell Biology·Y Gaudin
Apr 24, 2020·Journal of Virology·Venice Du PontRichard K Plemper
Feb 23, 2012·Viruses·Aurélie A V AlbertiniYves Gaudin
Mar 27, 2004·Nature Reviews. Microbiology·Dimiter S Dimitrov
Dec 16, 1998·Journal of Virology·C JalletP Perrin
Mar 25, 2017·Macromolecular Bioscience·Sydney HollingsheadJulie C Liu
Jul 3, 2002·Chemistry and Physics of Lipids·Robert BlumenthalAnu Puri
Oct 29, 2013·Journal of Medicinal Chemistry·Marco CantisaniStefania Galdiero
Feb 10, 2007·Science·Stéphane RocheStéphane Bressanelli

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