Biological functionality of extracellular matrix-ornamented three-dimensional printed hydroxyapatite scaffolds

Journal of Biomedical Materials Research. Part a
A KumarR D K Misra

Abstract

Three-dimensional (3D) printing is considered an ideally suitable method to fabricate patient specific implantable devices. The approach enabled to produce a porous scaffold with tailored physical, mechanical, and biological properties because of the flexibility to tune the scaffold architecture. The objective of the study described was to elucidate the determining role of cell-laid extracellular matrix (ECM) in impacting biological response. In this regard, to mimic the natural ECM environment or the attributes of the native tissue, a natural ECM analogue surface was produced on the 3D printed and sintered hydroxyapatite (HA) scaffold surface by the mineralized ECM of the osteoblast. This involved the growth of osteoblast on 3D printed scaffolds, followed by differentiation to deposit the mineralized ECM on the biomaterial surface. The cells were removed from the mineralized matrix using freeze-thaw cycles to obtain a decellularized extracellular matrix (dECM) on the biomaterial surface. Subsequently, seeding of osteoblast on dECM-ornamented HA scaffolds led to 3D growth with enhanced expression of prominent proteins, actin and vinculin. Based on preliminary observations of present study, it was underscored that HA scaffolds-o...Continue Reading

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Citations

Jun 28, 2016·Materials·Vuk Uskoković, Victoria M Wu
Dec 21, 2016·Advanced Healthcare Materials·Haitao CuiLijie Grace Zhang
Jan 27, 2019·Bioengineering & Translational Medicine·Yu Seon KimAntonios G Mikos
Dec 17, 2016·Tissue Engineering and Regenerative Medicine·Sang-Hyug ParkByoung-Hyun Min
Feb 25, 2020·Journal of the Mechanical Behavior of Biomedical Materials·Raffaella PecciCostantino Del Gaudio
Jan 1, 2021·Frontiers in Bioengineering and Biotechnology·Yongtao ZhangMostafa El Dafrawy
Aug 29, 2021·Differentiation; Research in Biological Diversity·Mohammadreza SafdariJavad Hashemi

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