Biological hydrogen production using chloroform-treated methanogenic granules

Applied Biochemistry and Biotechnology
Bo Hu, S Chen

Abstract

In fermentative hydrogen production, the low-hydrogen-producing bacteria retention rate limits the suspended growth reactor productivity because of the long hydraulic retention time (HRT) required to maintain adequate bacteria population. Traditional bacteria immobilization methods such as calcium alginate entrapment have many application limitations in hydrogen fermentation, including limited duration time, bacteria leakage, cost, and so on. The use of chloroform-treated anaerobic granular sludge as immobilized hydrogen-producing bacteria in an immobilized hydrogen culture may be able to overcome the limitations of traditional immobilization methods. This paper reports the findings on the performance of fed-batch cultures and continuous cultures inoculated with chloroform-treated granules. The chloroform-treated granules were able to be reused over four fed-batch cultures, with pH adjustment. The upflow reactor packed with chloroform-treated granules was studied, and the HRT of the upflow reactor was found to be as low as 4 h without any decrease in hydrogen production yield. Initial pH and glucose concentration of the culture medium significantly influenced the performance of the reactor. The optimum initial pH of the culture...Continue Reading

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Citations

Jan 16, 2016·International Journal of Molecular Sciences·Vinayak Laxman PachapurAntonio Avalos Ramirez
Apr 17, 2015·Journal of Environmental Management·M A Zumar BundhooM Ali Hassan
Aug 3, 2016·Journal of Anesthesia History·Theodore A Alston

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