Biological interaction levels of zinc oxide nanoparticles; lettuce seeds as case study

Heliyon
Rabeah Yousef RawashdehAsma Mahmoud AlHasan

Abstract

Seed germination is a critical stage in plant life, and recent practices use nanomaterials for the improvement of plant seed germination indices. This study was conducted to assess the effect of laboratory prepared zinc oxide nanoparticles on the physiological and biochemical changes of lettuce seeds. Lettuce seeds were soaked in a suspension of moderately polydisperse zinc oxide nanoparticles at two different concentrations (25 ppm or 50 ppm) and shaken for 3 h at 25 °C. Seeds treatment was followed subsequently by two to three days drying at ambient conditions. Treated seeds were stored for 3-4 weeks, at ambient conditions and then tested for germination in petri dishes. Germination was observed on daily basis and seedling length was measured. After imbibition and before the start of the visible germination, seeds were examined for topography and surface analysis using the scanning electron microscope and zinc uptake was measured by using the atomic absorption spectrometry and the energy dispersive X-ray. The pattern of mobilization of biomolecules was analyzed to detect any differences among different seed groups. There was no loss of viability for the nanoparticles treated seeds. Indeed their germination was enhanced and th...Continue Reading

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Methods Mentioned

BETA
Atomic absorption spectrometry
dynamic light scattering
X-ray

Software Mentioned

SPSS

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