Biological properties of lipid A isolated from Flavobacterium meningosepticum

Clinical and Diagnostic Laboratory Immunology
K TanamotoS Azumi

Abstract

The biological properties of the lipid A from Flavobacterium meningosepticum, which we recently isolated and whose complete chemical structure has been determined (H. Kato, T. Iida, Y. Haishima, A. Tanaka, and K. Tanamoto. J. Bacteriol. 180:3891--3899, 1998), were studied. The lipid A exhibited generally moderate activity compared to Salmonella enterica subsp. enterica serovar abortus equi lipopolysaccharide (LPS) used as a control in the assay systems tested; lethal toxicity in galactosamine-sensitized mice, mitogenicity in mouse spleen cells, induction of tumor necrosis factor alpha (TNF-alpha) release from mouse peritoneal macrophages and J774-1 mouse macrophage-like and human THP-1 line cells, nitric oxide induction activity from J774-1 cells, and Limulus gelation activity. The moderate activity of the F. meningosepticum lipid A may be explained by its unique fatty acid composition and the lack of a phosphate group in position 4'. It is noteworthy that the lipid A apparently induced TNF-alpha release from peritoneal macrophages in LPS-unresponsive C3H/HeJ mice and that the activation was suppressed by the LPS-specific antagonist, succinylated lipid A precursor. Significant splenocyte mitogenicity in C3H/HeJ mice was also ob...Continue Reading

References

Sep 1, 1976·The Journal of Experimental Medicine·D C MorrisonD M Jacobs
Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C GalanosW Reutter
Sep 1, 1976·The Journal of Experimental Medicine·B M Sultzer, G W Goodman
Apr 1, 1989·European Journal of Biochemistry·J H KraussH Mayer
Jan 1, 1986·American Journal of Surgery·G B BulkleyR W Bailey
Jun 1, 1969·European Journal of Biochemistry·C GalanosO Westphal
Jul 1, 1983·Journal of Bacteriology·W StrittmatterC Galanos
Oct 1, 1982·Analytical Biochemistry·L C GreenS R Tannenbaum
Jan 1, 1997·Microbiology·Ken-Ichi TanamotoToshio Umemoto
Mar 8, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·K Tanamoto, S Azumi

❮ Previous
Next ❯

Citations

Feb 27, 2010·Journal of the American Society for Mass Spectrometry·Jace W JonesRobert K Ernst
Jul 14, 2010·Biochemistry. Biokhimii︠a︡·D S Kabanov, I R Prokhorenko
Nov 17, 2010·Biochemistry. Biokhimii︠a︡·A I TukhvatulinA L Gintsburg
Mar 28, 2020·Journal of Applied Microbiology·Natalya S VelichkoYulia P Fedonenko
Oct 30, 2007·Journal of Medical Microbiology·Yutaka MakimuraTomohiko Ogawa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.