PMID: 30141589Mar 1, 2016Paper

Biological Properties of Nocardia vaccinii IMV B-7405 Surfactants Synthesized on Fried Sunflower Oil

Mikrobiolohichnyĭ zhurnal. = Mikrobiologichny zhurnal
T P PirogG O Iutynska

Abstract

To study of antimicrobial and antiadhesive properties of the surface-active substances (surfactants) Nocardia vaccinii IMV B-7405 synthesized on different oil-containing substrates (refined and waste after frying meat and potatoes sunflower oil). Surfactants were extracted from supernatant of cultural liquid by mixture of chloroform and methanol (2 : 1). Antimicrobial against bacteria and yeast properties of the surfactant was determined by index of the minimum inhibitory concentration (MIC). The number (%) of attached cells (adhesion) was determined as a ratio of the optical density of the suspension obtained from the materials treated with surfactants to the optical density of the control samples (100 %). It was established that surfactants synthesized N. vaccinii IMV B-7405 on fried oil, at a concentration of 0.02 - 0.04 mg/ml decreased adhesion of bacteria (Escherichia coli IEM-1, Bacillus subtilis BT-2) on plastic, dutch tile, glass and linoleum by 25-90 %, and yeast Candida albicans Д-6 - by 15 - 65 %. The minimum inhibitory concentration with respect to studied test cultures surfactants synthesized on waste after frying potato oil was 8 - 67 mg/ml, and against phytopathogenic bacteria Pectobacterium carotovorum UCM B-109...Continue Reading

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