PMID: 30141859Sep 1, 2016Paper

Biological Properties of Nocardia vaccinii IMV B-7405 Surfactants Synthesized on Byproduct of Biodiesel Production

Mikrobiolohichnyĭ zhurnal. = Mikrobiologichny zhurnal
T P PirogG O Iutynska

Abstract

Comparison of antimicrobial and anti-adhesive activity of Nocardia vaccinii ІMV B-7405 surfactants synthesized on purified and technical glycerol (byproduct of biodiesel production). Surfactants were extracted from supernatant of cultural liquid by mixture of chloroform and methanol (2:1). Antimicrobial against bacteria and yeast properties of the surfactant was determined by index of the minimum inhibitory concentration (MIC). The number of attached cells was determined spectrophotometrically. The dependence of surfactant antimicrobial and anti-aghesive activity on the degree of glycerol purification (purified, technical), as well as the duration of N. vaccinii IMV B-7405 cultivation on these substrates was established. MIC against studied test cultures surfactants, synthesized on technical glycerol during 5 days, was 15–121 μg/ml, that lower than MIC of surfactant obtained on purified substrate (22.5−180 μg/ml). Increasing duration of N. vaccinii ІMV B-7405 cultivation accompanied by rise the MIC against some test cultures surfactants synthesized on both technical and purified glycerol. Adhesion of the bacteria Escherichia coli ІEM-1, Bacillus subtilis BT-2 (vegetative cells and spores) and the yeast Candida albicans Д-6 on a...Continue Reading

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