Biological response of an in vitro human 3D lung cell model exposed to brake wear debris varies based on brake pad formulation

Archives of Toxicology
H BarošováBarbara Rothen-Rutishauser

Abstract

Wear particles from automotive friction brake pads of various sizes, morphology, and chemical composition are significant contributors towards particulate matter. Knowledge concerning the potential adverse effects following inhalation exposure to brake wear debris is limited. Our aim was, therefore, to generate brake wear particles released from commercial low-metallic and non-asbestos organic automotive brake pads used in mid-size passenger cars by a full-scale brake dynamometer with an environmental chamber simulating urban driving and to deduce their potential hazard in vitro. The collected fractions were analysed using scanning electron microscopy via energy-dispersive X-ray spectroscopy (SEM-EDS) and Raman microspectroscopy. The biological impact of the samples was investigated using a human 3D multicellular model consisting of human epithelial cells (A549) and human primary immune cells (macrophages and dendritic cells) mimicking the human epithelial tissue barrier. The viability, morphology, oxidative stress, and (pro-)inflammatory response of the cells were assessed following 24 h exposure to ~ 12, ~ 24, and ~ 48 µg/cm2 of non-airborne samples and to ~ 3.7 µg/cm2 of different brake wear size fractions (2-4, 1-2, and 0.2...Continue Reading

References

Apr 1, 1997·American Journal of Respiratory and Critical Care Medicine·A PetersJ Heyder
Dec 15, 2000·The New England Journal of Medicine·J M SametS L Zeger
Mar 7, 2002·JAMA : the Journal of the American Medical Association·C Arden PopeGeorge D Thurston
Oct 4, 2003·Environmental Science & Technology·Paul G SandersM Matti Maricq
Mar 3, 2004·American Journal of Industrial Medicine·Richard A Lemen
Jul 2, 2004·American Journal of Respiratory and Critical Care Medicine·Frank SchaumannNorbert Krug
Jan 11, 2005·American Journal of Respiratory Cell and Molecular Biology·Barbara M Rothen-RutishauserPeter Gehr
Jan 18, 2005·Chemical Research in Toxicology·Hanna L KarlssonLennart Möller
Mar 3, 2005·Journal of Aerosol Medicine : the Official Journal of the International Society for Aerosols in Medicine·Holger SchulzAnnette Peters
Jul 9, 2005·Environmental Health Perspectives·Günter OberdörsterJan Oberdörster
Oct 1, 2005·Inhalation Toxicology·Andrew J Ghio, Mitchell D Cohen
Oct 13, 2006·Journal of Aerosol Medicine : the Official Journal of the International Society for Aerosols in Medicine·Fabian BlankPeter Gehr
Feb 3, 2007·American Journal of Respiratory Cell and Molecular Biology·Fabian BlankPeter Gehr
Jul 19, 2008·The Science of the Total Environment·Alistair Thorpe, Roy M Harrison
Aug 6, 2008·Expert Opinion on Drug Metabolism & Toxicology·Barbara Rothen-RutishauserPeter Gehr
Apr 21, 2009·Particle and Fibre Toxicology·Shrilatha BalakrishnaStephania A Cormier
Nov 26, 2009·Particle and Fibre Toxicology·Michael GasserBarbara Rothen-Rutishauser
Dec 18, 2009·Particle and Fibre Toxicology·Anke Gabriele LenzOtmar Schmid
Mar 9, 2010·Particle and Fibre Toxicology·Marianne Geiser, Wolfgang G Kreyling
Jun 30, 2010·Circulation·Qinghua SunLoren E Wold
Oct 27, 2010·Proceedings of the National Academy of Sciences of the United States of America·Amir S YazdiJürg Tschopp
Jan 21, 2011·Environmental Pollution·Jana KukutschováPeter Filip
Jun 26, 2014·Experimental Biology and Medicine·Joan E NicholsJoaquin Cortiella
Sep 24, 2014·Particle and Fibre Toxicology·Carola EndesMartin J D Clift
Oct 17, 2014·Environmental Science and Pollution Research International·Theodoros Grigoratos, Giorgio Martini
May 18, 2016·Ecotoxicology and Environmental Safety·Katerina MalachovaPeter Filip
Oct 12, 2017·Environmental Health Perspectives·Vicki StoneFlemming R Cassee

❮ Previous
Next ❯

Citations

Jul 28, 2021·Environmental Science & Technology·Kohei SakataHiroshi Tanimoto
Aug 6, 2019·Environmental Science & Technology·Tomasz Gonet, Barbara A Maher

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
protein assay
enzyme-linked immunosorbent assay
ELISA

Software Mentioned

GraphPad Prism
IMARIS
GraphPad

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.