Biological Response to Macroporous Chitosan-Agarose Bone Scaffolds Comprising Mg- and Zn-Doped Nano-Hydroxyapatite

International Journal of Molecular Sciences
Paulina KazimierczakAgata Przekora

Abstract

Modification of implantable scaffolds with magnesium and zinc for improvement of bone regeneration is a growing trend in the engineering of biomaterials. The aim of this study was to synthesize nano-hydroxyapatite substituted with magnesium (Mg2+) (HA-Mg) and zinc (Zn2+) (HA-Zn) ions in order to fabricate chitosan-agarose-hydroxyapatite (HA) scaffolds (chit/aga/HA) with improved biocompatibility. Fabricated biomaterials containing Mg2+ or Zn2+ were tested using osteoblasts and mesenchymal stem cells to determine the effect of incorporated metal ions on cell adhesion, spreading, proliferation, and osteogenic differentiation. The study was conducted in direct contact with the scaffolds (cells were seeded onto the biomaterials) and using fluid extracts of the materials. It demonstrated that incorporation of Mg2+ ions into chit/aga/HA structure increased spreading of the osteoblasts, promoted cell proliferation on the scaffold surface, and enhanced osteocalcin production by mesenchymal stem cells. Although biomaterial containing Zn2+ did not improve cell proliferation, it did enhance type I collagen production by mesenchymal stem cells and extracellular matrix mineralization as compared to cells cultured in a polystyrene well. Neve...Continue Reading

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Citations

Jan 28, 2021·International Journal of Molecular Sciences·Paulina KazimierczakAgata Przekora
Feb 20, 2021·International Journal of Nanomedicine·Salma El-HabashyAmal H El-Kamel
Mar 15, 2021·Biological Trace Element Research·Eda Çiftci DedeFeza Korkusuz

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Methods Mentioned

BETA
X-ray
infrared spectroscopy
enzyme-linked immunosorbent assays
inductively coupled plasma spectrometry
Assay
ELISA
Protein Assay

Software Mentioned

GraphPad Prism
TOPAS
Doubling Time Computing
ImageJ

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