Biological Sources of Intrinsic and Extrinsic Noise in cI Expression of Lysogenic Phage Lambda

Scientific Reports
Xue LeiPing Ao

Abstract

Genetically identical cells exposed to homogeneous environment can show remarkable phenotypic difference. To predict how phenotype is shaped, understanding of how each factor contributes is required. During gene expression processes, noise could arise either intrinsically in biochemical processes of gene expression or extrinsically from other cellular processes such as cell growth. In this work, important noise sources in gene expression of phage λ lysogen are quantified using models described by stochastic differential equations (SDEs). Results show that DNA looping has sophisticated impacts on gene expression noise: When DNA looping provides autorepression, like in wild type, it reduces noise in the system; When the autorepression is defected as it is in certain mutants, DNA looping increases expression noise. We also study how each gene operator affects the expression noise by changing the binding affinity between the gene and the transcription factor systematically. We find that the system shows extraordinarily large noise when the binding affinity is in certain range, which changes the system from monostable to bistable. In addition, we find that cell growth causes non-negligible noise, which increases with gene expression...Continue Reading

References

Jan 20, 1985·Journal of Molecular Biology·M A Shea, G K Ackers
Aug 3, 1999·The EMBO Journal·J W LittleD W Wert
Jun 13, 2000·Nature·A Becskei, L Serrano
Jun 15, 2000·Proceedings of the National Academy of Sciences of the United States of America·J PaulssonM Ehrenberg
Apr 23, 2002·Nature Genetics·Ertugrul M OzbudakAlexander van Oudenaarden
Aug 17, 2002·Science·Michael B ElowitzPeter S Swain
Sep 19, 2002·Proceedings of the National Academy of Sciences of the United States of America·Peter S SwainEric D Siggia
Nov 15, 2002·Nature·Christopher V RaoAdam P Arkin
Apr 11, 2003·Nature·William J BlakeJ J Collins
Jun 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Farren J IsaacsJ J Collins
Aug 21, 2003·Journal of Molecular Biology·José M G Vilar, Stanislas Leibler
May 29, 2004·Science·Jonathan M Raser, Erin K O'Shea
Oct 19, 2004·Journal of Theoretical Biology·Audun Bakk, Ralf Metzler
Dec 24, 2004·Journal of Bioinformatics and Computational Biology·X-M ZhuP Ao
Mar 26, 2005·Science·Nitzan RosenfeldMichael B Elowitz
May 11, 2005·Nature Reviews. Genetics·Mads KaernJames J Collins
Sep 6, 2005·Proceedings of the National Academy of Sciences of the United States of America·Chulan KwonDavid J Thouless
Dec 24, 2005·Nature·Dmitri VolfsonJeff Hasty
Feb 17, 2006·Nature·Nicholas J GuidoJ J Collins
Dec 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Shota Atsumi, John W Little
Jun 16, 2007·Science·Hédia MaamarDavid Dubnau
Jan 19, 2008·Science·Juan M Pedraza, Johan Paulsson
Apr 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·L Meadow Anderson, Haw Yang
May 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Marco J MorelliRosalind J Allen
Jul 2, 2009·Physical Biology·Saburo TsuruTetsuya Yomo
Oct 8, 2009·Physical Biology·Maria Werner, Erik Aurell
Sep 11, 2010·Nature·Avigdor Eldar, Michael B Elowitz
Sep 28, 2010·Journal of Bacteriology·John W Little, Christine B Michalowski
Oct 13, 2010·Proceedings of the National Academy of Sciences of the United States of America·Youfang CaoJie Liang
Mar 11, 2011·PLoS Computational Biology·Alvaro SanchezJané Kondev
Jul 4, 2012·Nature Structural & Molecular Biology·Zach HenselJie Xiao
Feb 6, 2013·Proceedings of the National Academy of Sciences of the United States of America·Lun CuiIan B Dodd
Apr 23, 2013·Biophysical Journal·Michał KomorowskiMichael P H Stumpf
Nov 6, 2013·Bioinformatics·Tomasz JetkaMichał Komorowski
Dec 7, 2013·Science·Alvaro Sanchez, Ido Golding

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