PMID: 16646489May 2, 2006Paper

Biological validation of a sample preparation method for ER-CALUX bioanalysis of estrogenic activity in sediment using mixtures of xeno-estrogens

Environmental Science & Technology
Corine J HoutmanJ Legler

Abstract

The combined estrogenic effects of mixtures of environmental pollutants in the in vitro ER-CALUX (chemical activated luciferase gene expression) bioassaywere examined to biologically validate a sample preparation method for the analysis of estrogenic compounds in sediment. The method used accelerated solvent extraction (ASE) and gel permeation chromatography (GPC) and was validated with respect to recovery of biological response taking mixture effects into account. Four mixtures of three to six xenoestrogenic compounds (bisphenol A, 4-nonylphenol, (4,4'-dichlorodiphenyl)trichloroethane, (2,4'-dichlorodiphenyl)trichloroethane, dieldrin, 4-n-octylphenol, alpha-chlordane, dibutylphthalate, (4,4'-dichlorodiphenyl)dichloroethylene, and 2,4,5-trichlorobiphenyl) were prepared. Experimentally determined mixture effects were well described by the concept of concentration addition (CA), as expected for similarly acting compounds. Observed estradiol equivalence factors of the mixtures (on average 1.2 +/- 0.3) agreed very well with the value predicted according to CA. The sample preparation method was then applied to pure mixtures of standards and to sediment spiked with one of the mixtures. Recoveries of estrogenic compounds were estimate...Continue Reading

References

May 20, 1999·Toxicological Sciences : an Official Journal of the Society of Toxicology·J LeglerB van der Burg
Sep 24, 1999·The Science of the Total Environment·A Kortenkamp, R Altenburger
May 12, 2000·The Science of the Total Environment·J PayneA Kortenkamp
Oct 26, 2000·Environmental Health Perspectives·J PayneA Kortenkamp
Mar 15, 2001·Toxicological Sciences : an Official Journal of the Society of Toxicology·N RajapakseA Kortenkamp
May 4, 2001·Environmental Health Perspectives·J PayneA Kortenkamp
Jul 13, 2002·Archives of Environmental Contamination and Toxicology·K HilscherovaJ P Giesy
Oct 22, 2002·Environmental Science & Technology·Juliette LeglerBart Van der Burg
Feb 11, 2004·Environmental Toxicology and Chemistry·Corine J HoutmanAbraham Brouwer
Apr 16, 2004·Toxicological Sciences : an Official Journal of the Society of Toxicology·Chris GenningsRichard A Carchman
Dec 16, 2004·Environmental Science & Technology·Corine J HoutmanJuliette Legler

❮ Previous
Next ❯

Citations

Jan 29, 2008·Analytical and Bioanalytical Chemistry·Werner BrackTobias Schulze
Aug 17, 2010·Ecotoxicology·Hui ZhangHong-Er Tian
Jul 10, 2007·The Science of the Total Environment·Corine J HoutmanHans J C Klamer
Nov 1, 2007·International Journal of Andrology·Bart van der BurgEdwin Sonneveld
Jul 12, 2016·Environmental Science and Pollution Research International·Linda GehrmannJochen Tuerk
Aug 3, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Heidi DemaegdtKarine Vandermeiren
Jan 2, 2020·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Pei-Hsin ChouYi-Po Yeh
Apr 15, 2014·Environmental Science & Technology·Markus BrinkmannHenner Hollert

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.