Biologically active interleukin 1 in human eccrine sweat: site-dependent variations in alpha/beta ratios and stress-induced increased excretion

Cytokine
L DidierjeanJ H Saurat

Abstract

Human eccrine sweat devoid of epidermal protein contamination was collected from palms, soles, and different sites on the trunk. Interleukin 1 alpha (IL 1 alpha) and interleukin 1 beta (IL 1 beta) content were analyzed for immunoreactivity by enzymo-immunoassay and immunoblotting and for bioactivity by the stimulation of prostaglandin E2 (PGE2) production in human dermal fibroblasts. The bioassay was validated by using blocking antibodies against IL 1 alpha and beta. All sweat samples were found to contain significant amounts of immunoreactive and biologically active IL 1. The immunoreactive forms were at 17 kDa as shown by immunoblotting analysis, indicating that they were mature (secreted), undegraded IL 1 peptides. Whereas IL 1 alpha was detectable in sweat samples obtained from both truncal and palmo-plantar regions, IL 1 beta was only detectable in the sweat of palms and soles (IL 1 alpha/beta ratio greater than 700 in trunk and 5.4 in palms and soles) indicating a site-dependent difference in the excretion of the two IL 1 molecules. IL 1 concentration was high in spontaneous (IL 1 alpha, 3.7; IL 1 beta, 0.3 ng/mL) and pilocarpine induced sweat (IL 1 alpha, 3.9; IL 1 beta, 1.2 ng/mL), and it was much increased during joggi...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Apr 1, 1987·The Journal of Investigative Dermatology·T V BellD N Sauder
Oct 1, 1985·The Journal of Clinical Investigation·L C GahringR A Daynes
Nov 1, 1988·Immunology Today·L Fitzgerald
Aug 1, 1987·The Journal of Clinical Investigation·T S KupperU Gubler
Dec 1, 1973·Analytical Biochemistry·W Schaffner, C Weissmann
Jan 5, 1972·Nature: New Biology·E Frömter, J Diamond
Dec 1, 1965·The Journal of Investigative Dermatology·P Williamson, A M Kligman
May 1, 1984·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·T C BoysenK Sato
Apr 21, 1983·The New England Journal of Medicine·E Atkins
May 6, 1983·Science·J G Cannon, M J Kluger
Sep 1, 1981·Survey of Ophthalmology·N J Van Haeringen
Jul 1, 1961·Biochemical Pharmacology·G L ELLMANR M FEATHER-STONE
Jan 1, 1987·Immunology Today·S K Dower, D L Urdal

❮ Previous
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Citations

Jan 1, 1996·Pathology Oncology Research : POR·Antal Rot
Oct 16, 2002·Journal of Immunological Methods·Thomas FladHubert Kalbacher
Dec 1, 1992·The British Journal of Dermatology·H S AnttilaJ H Saurat
Sep 3, 2003·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Yan-Wu Tian, Michael C Stacey
Oct 3, 2007·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·Cindy M de JonghSanja Kezic
Nov 6, 2001·Nature Immunology·B SchittekC Garbe
Dec 2, 1991·FEBS Letters·D Gruaz-ChatellardJ M Dayer
Jul 22, 2018·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·Karan AgrawalJohn W Newman
Mar 4, 2020·European Journal of Applied Physiology·Lindsay B Baker, Anthony S Wolfe
Dec 5, 2018·Archives of Dermatological Research·Yoshiko MizukawaTetsuo Shiohara
Sep 25, 2017·Prostaglandins & Other Lipid Mediators·Karan AgrawalJohn W Newman
Oct 9, 2002·Progress in Histochemistry and Cytochemistry·Kenji Saga

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