Bioluminescence color modulation of Vibrio fischeri strain Y1 coupled with alterable levels of endogenous yellow fluorescent protein and its fluorescence imaging

Photochemistry and Photobiology
H KarataniSatoshi Hirayama

Abstract

Bioluminescence (BL) (lambda(max) approximately 535 nm) of Vibrio fischeri strain Y1 has been previously characterized in terms of the fluctuation in intracellular levels of yellow fluorescent protein (YFP). In this study fluorescence microscopic analysis has revealed that yellow fluorescence, as well as blue fluorescence attributable to a luciferase intermediate, is localized to the periphery of V. fischeri Y1 cells. This finding indicates that both YFP and the luciferase are present in the vicinity of the cell membrane. By using cyanide to enhance yellow BL, it has been shown that BL modulation is coupled with the fluctuations in the intracellular levels of YFP and the primary emitter. On the basis of the BL characterization, combined with results of a sedimentation experiment, it has been shown that larger cells produce a relatively stronger yellow BL. Two-dimensional gel electrophoresis of cell-protein extracts has shown that the YFP level is more alterable than the luciferase level. It is postulated that the yellow BL modulation takes place in connection with cell growth.

References

Apr 22, 1977·Science·E G Ruby, K H Nealson
Oct 21, 1975·Biochemistry·C Balny, J W Hastings
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S C DaubnerT O Baldwin
Jul 15, 1987·Biochemical and Biophysical Research Communications·P MacherouxJ W Hastings
Jan 1, 1985·Advances in Microbial Physiology·J W HastingsJ C Makemson
May 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·K H NealsonJ W Hastings
Oct 1, 1970·Journal of Bacteriology·K H NealsonJ W Hastings
Aug 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·E A Meighen
Feb 21, 2004·Photochemistry and Photobiology·Hajime KarataniSatoshi Hirayama
Feb 1, 1980·Applied and Environmental Microbiology·E G RubyJ W Hastings
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M KurfürstJ W Hastings

❮ Previous
Next ❯

Citations

Jun 19, 2007·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hajime Karatani
Mar 16, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Hajime KarataniHiroshi Oyama

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence
electrophoresis
protein assay
fluorescence imaging

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology
H KarataniSatoshi Hirayama
© 2022 Meta ULC. All rights reserved