Bioluminescent Antibodies for Point-of-Care Diagnostics

Angewandte Chemie
Lin XueKai Johnsson

Abstract

We introduce a general method to transform antibodies into ratiometric, bioluminescent sensor proteins for the no-wash quantification of analytes. Our approach is based on the genetic fusion of antibody fragments to NanoLuc luciferase and SNAP-tag, the latter being labeled with a synthetic fluorescent competitor of the antigen. Binding of the antigen, here synthetic drugs, by the sensor displaces the tethered fluorescent competitor from the antibody and disrupts bioluminescent resonance energy transfer (BRET) between the luciferase and fluorophore. The semisynthetic sensors display a tunable response range (submicromolar to submillimolar) and large dynamic range (ΔRmax >500 %), and they permit the quantification of analytes through spotting of the samples onto paper followed by analysis with a digital camera.

References

Jul 1, 1979·Clinical Pharmacology and Therapeutics·R C DonehowerB A Chabner
Aug 20, 1987·Journal of Molecular Biology·C Chothia, A M Lesk
Jul 31, 1999·FEBS Letters·H ShiraiH Nakamura
Sep 5, 2003·Cancer Biotherapy & Radiopharmaceuticals·Barbara H BielaAlan L Epstein
Sep 10, 2005·Nature Biotechnology·Philipp Holliger, Peter J Hudson
May 24, 2007·Analytical and Bioanalytical Chemistry·Franck Saint-MarcouxPierre Marquet
Jan 30, 2008·Biotechnology Journal·Johan BacartCyril Couturier
Mar 8, 2011·Journal of Pharmaceutical and Biomedical Analysis·James HeatonNorman W Smith
Sep 2, 2011·Journal of the American Chemical Society·Matthias A BrunKai Johnsson
Jul 28, 2012·Critical Care : the Official Journal of the Critical Care Forum·Martin SchulzAchim Schmoldt
May 17, 2014·Biochemistry·Susan GaydaSergey Y Tetin
Jun 9, 2014·Nature Chemical Biology·Rudolf GrissKai Johnsson
Nov 25, 2014·Cell·Keith PardeeJames J Collins
Mar 15, 2015·Frontiers in Bioengineering and Biotechnology·Barbara Sanavio, Silke Krol
Jul 16, 2015·MAbs·Alexander BujotzekGuy Georges
Mar 25, 2017·Methods in Enzymology·Remco ArtsMaarten Merkx

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Citations

Sep 1, 2018·Angewandte Chemie·Keisuke TendaDaniel Citterio
Jul 28, 2020·Chemical Communications : Chem Comm·Dongdong XuSeraphine V Wegner
Feb 21, 2019·Annual Review of Analytical Chemistry·Hsien-Wei Yeh, Hui-Wang Ai
Nov 24, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Meng-Meng PanLi Xu
Nov 27, 2020·Biochemical Society Transactions·Lieuwe BiewengaMaarten Merkx
Mar 16, 2019·Current Opinion in Chemical Biology·Seiji SakamotoItaru Hamachi
Jun 8, 2018·Current Opinion in Chemical Biology·Zi YaoJennifer A Prescher
Mar 19, 2021·Chemical Society Reviews·Aisha J Syed, James C Anderson
Mar 13, 2021·Angewandte Chemie·Emmanuel DelamarcheSimone Schuerle
Dec 10, 2020·Trends in Biotechnology·Alexander Gräwe, Viktor Stein
May 27, 2021·Biosensors & Bioelectronics·Jun Jie OngAbdul W Basit
Jun 15, 2021·Chemical Reviews·Eka NovianaCharles S Henry
Jun 7, 2018·ACS Sensors·Jin-Wen ShangguanHong-Yuan Chen
Feb 13, 2018·ACS Sensors·Tsuyoshi UedaItaru Hamachi

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Methods Mentioned

BETA
biosensors
BRET
biosensor

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