Bioluminescent Reporting of In Vivo IFN-γ Immune Responses during Infection and Autoimmunity

The Journal of Immunology : Official Journal of the American Association of Immunologists
Catherine J ReynoldsRosemary J Boyton

Abstract

IFN-γ is a key cytokine of innate and adaptive immunity. It is important to understand temporal changes in IFN-γ production and how these changes relate to the role of IFN-γ in diverse models of infectious and autoimmune disease, making the ability to monitor and track IFN-γ production in vivo of a substantial benefit. IFN-γ ELISPOTs have been a central methodology to measure T cell immunity for many years. In this study, we add the capacity to analyze IFN-γ responses with high sensitivity and specificity, longitudinally, in vitro and in vivo. This allows the refinement of experimental protocols because immunity can be tracked in real-time through a longitudinal approach. We have generated a novel murine IFN-γ reporter transgenic model that allows IFN-γ production to be visualized and quantified in vitro and in vivo as bioluminescence using an imaging system. At baseline, in the absence of an inflammatory stimulus, IFN-γ signal from lymphoid tissue is detectable in vivo. Reporter transgenics are used in this study to track the IFN-γ response to Pseudomonas aeruginosa infection in the lung over time in vivo. The longitudinal development of the adaptive T cell immunity following immunization with Ag is identified from day 7 in vi...Continue Reading

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Citations

Oct 16, 2019·Proceedings of the National Academy of Sciences of the United States of America·Haiguang WangKristin A Hogquist
Nov 5, 2021·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Chintan ChawdaLaura Mezzanotte
Nov 9, 2021·Cell Structure and Function·Taisei TanakaKenta Terai

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Methods Mentioned

BETA
transgenic
PCR
biopsy
FCS
PMA
ELISA
flow cytometry
transgenics

Software Mentioned

ELISPOT
Living Image

Related Concepts

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