Bioluminescent response of individual dinoflagellate cells to hydrodynamic stress measured with millisecond resolution in a microfluidic device

The Journal of Experimental Biology
Michael I LatzAlex Groisman

Abstract

Dinoflagellate bioluminescence serves as a model system for examining mechanosensing by suspended motile unicellular organisms. The response latency, i.e. the delay time between the mechanical stimulus and luminescent response, provides information about the mechanotransduction and signaling process, and must be accurately known for dinoflagellate bioluminescence to be used as a flow visualization tool. This study used a novel microfluidic device to measure the response latency of a large number of individual dinoflagellates with a resolution of a few milliseconds. Suspended cells of several dinoflagellate species approximately 35 microm in diameter were directed through a 200 microm deep channel to a barrier with a 15 microm clearance impassable to the cells. Bioluminescence was stimulated when cells encountered the barrier and experienced an abrupt increase in hydrodynamic drag, and was imaged using high numerical aperture optics and a high-speed low-light video system. The average response latency for Lingulodinium polyedrum strain HJ was 15 ms (N>300 cells) at the three highest flow rates tested, with a minimum latency of 12 ms. Cells produced multiple flashes with an interval as short as 5 ms between individual flashes, su...Continue Reading

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Citations

Apr 8, 2009·Lab on a Chip·Eugene TkachenkoAlex Groisman
Dec 15, 2010·Annual Review of Marine Science·Steven H D HaddockJames F Case
Sep 5, 2013·Microorganisms·Martha Valiadi, Debora Iglesias-Rodriguez
Jul 12, 2019·Nature·Arnold J T M MathijssenManu Prakash
Mar 11, 2020·International Journal of Molecular Sciences·Carlos FajardoFrancisco Javier Fernandez-Acero
Jul 24, 2020·Physical Review Letters·Maziyar JalaalRaymond E Goldstein
Jan 14, 2011·The Journal of Experimental Biology·Houshuo Jiang, Thomas Kiørboe

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