Bioluminescent system for dynamic imaging of cell and animal behavior

Biochemical and Biophysical Research Communications
Chikako Hara-MiyauchiHideyuki Okano

Abstract

The current utility of bioluminescence imaging is constrained by a low photon yield that limits temporal sensitivity. Here, we describe an imaging method that uses a chemiluminescent/fluorescent protein, ffLuc-cp156, which consists of a yellow variant of Aequorea GFP and firefly luciferase. We report an improvement in photon yield by over three orders of magnitude over current bioluminescent systems. We imaged cellular movement at high resolution including neuronal growth cones and microglial cell protrusions. Transgenic ffLuc-cp156 mice enabled video-rate bioluminescence imaging of freely moving animals, which may provide a reliable assay for drug distribution in behaving animals for pre-clinical studies.

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Citations

May 29, 2012·Journal of the National Cancer Institute·Satsuki MochizukiYasunori Okada
Jan 23, 2016·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Masato YanoHideyuki Okano
Jun 22, 2015·Current Opinion in Chemical Biology·Kenta Saito, Takeharu Nagai
Feb 17, 2015·Laboratory Investigation; a Journal of Technical Methods and Pathology·Robert M Hoffman
Feb 22, 2018·Cancer Science·Takeshi ImamuraRyosuke Kawakami
Nov 4, 2017·Digestive Diseases and Sciences·Seiichiro FukuharaHidekazu Suzuki
May 8, 2019·Molecular Brain·Ryota TamuraMasahiro Toda
May 25, 2016·Chemistry : a European Journal·Shuji IokaShigeru Nishiyama
May 15, 2016·Differentiation; Research in Biological Diversity·Takehito OuchiHideyuki Okano

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