Biomarker and histopathological responses of Lates calcarifer on exposure to sub lethal concentrations of chlorpyrifos

Ecotoxicology and Environmental Safety
S R MarigoudarP Karthikeyan

Abstract

Bioassay tests on fingerlings (3.0 ± 0.5cm) of euryhyaline fish Lates calcarifer were conducted using customized continuous flow through system and derived 96h acute toxicity value for chlorpyrifos (CPF). Based on the measured concentration of CPF mean median lethal concentration (LC50) of 1.07µg/l with lower and upper 95% confidence limits (0.95 and 1.19µg/l). No observed Effect Concentration (NOEC), Lowest Observed Effect Concentration (LOEC) and chronic values were found to be 0.4, 0.09 and 0.07µg/l respectively. Key biomarker enzyme activities such as esterase, superoxide dismutase and malate dehydrogenase were measured in whole body tissues of the fish fingerlings on exposure to sublethal toxicity of CPF resulting in inhibition of enzyme activities. Native gel electrophoresis revealed single isoform of SOD and MDH enzyme activities exhibiting time and concentration dependent inhibition. Interestingly, three isoforms of esterase activity were witnessed, two isoforms didn't show changes and one isoform was completely inhibited. The observed changes indicated continuous production of reactive oxygen species (ROS) in cells, affecting the integrity and function of cell membrane. Decreased MDH activity indicates reduction of ATP...Continue Reading

Citations

Jan 1, 2019·Environmental Sciences Europe·J B LegradiH Hollert
Sep 13, 2019·Water Environment Research : a Research Publication of the Water Environment Federation·Alan J MearnsNicolle Rutherford
Sep 29, 2018·Environmental Science and Pollution Research International·Chongnv WangXuming Pan
Apr 30, 2021·Environmental Pollution·Shandong MengRobby Stoks
Aug 18, 2020·Environmental Science & Technology·Shandong MengRobby Stoks
May 26, 2020·Ecotoxicology and Environmental Safety·Xiao HuangWeiyan Duan

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