Biomarker discovery by CE-MS enables sequence analysis via MS/MS with platform-independent separation.

Electrophoresis
Petra ZürbigHarald Mischak

Abstract

CE-MS is a successful proteomic platform for the definition of biomarkers in different body fluids. Besides the biomarker defining experimental parameters, CE migration time and molecular weight, especially biomarker's sequence identity is an indispensable cornerstone for deeper insights into the pathophysiological pathways of diseases or for made-to-measure therapeutic drug design. Therefore, this report presents a detailed discussion of different peptide sequencing platforms consisting of high performance separation method either coupled on-line or off-line to different MS/MS devices, such as MALDI-TOF-TOF, ESI-IT, ESI-QTOF and Fourier transform ion cyclotron resonance, for sequencing indicative peptides. This comparison demonstrates the unique feature of CE-MS technology to serve as a reliable basis for the assignment of peptide sequence data obtained using different separation MS/MS methods to the biomarker defining parameters, CE migration time and molecular weight. Discovery of potential biomarkers by CE-MS enables sequence analysis via MS/MS with platform-independent sample separation. This is due to the fact that the number of basic and neutral polar amino acids of biomarkers sequences distinctly correlates with their C...Continue Reading

References

Oct 29, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·R LehmannH M Liebich
Feb 5, 1998·Journal of Mass Spectrometry : JMS·J R Yates
Jan 15, 1999·Journal of the American Society for Mass Spectrometry·M RaidaW G Forssmann
Apr 2, 2002·Biochemical and Biophysical Research Communications·Haleem J IssaqDonna Felschow
Oct 5, 2002·Disease Markers·W Hayes McDonald, John R Yates
Mar 20, 2003·Journal of Proteome Research·James X PangGregory J Opitek
Dec 9, 2003·Electrophoresis·Philippe Schmitt-Kopplin, Moritz Frommberger
Jul 27, 2004·Electrophoresis·Javier Hernández-BorgesMatthias Pelzing
Jul 30, 2004·Clinical Science·Harald MischakDanilo Fliser
Sep 25, 2004·Mass Spectrometry Reviews·Bogdan Bogdanov, Richard D Smith
Oct 1, 2004·Analytical Chemistry·Harrison K MusyimiKermit K Murray
Oct 14, 2004·Journal of Mass Spectrometry : JMS·Lekha Sleno, Dietrich A Volmer
Oct 21, 2004·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Eva M WeissingerRaymond C Vanholder
Mar 19, 2005·Electrophoresis·Philippe Schmitt-Kopplin, Matthias Englmann
Jun 21, 2005·Electrophoresis·Danilo FliserHarald Mischak

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Citations

Jul 11, 2007·World Journal of Urology·Bruce A JulianJan Novak
Aug 19, 2007·World Journal of Urology·Dan Theodorescu, Harald Mischak
Jan 5, 2008·World Journal of Urology·Eric SchifferAntonia Vlahou
Dec 4, 2010·Journal of Proteome Research·Nagarjuna Nagaraj, Matthias Mann
Apr 5, 2007·Nature Protocols·Josep VillanuevaPaul Tempst
Aug 13, 2008·Proceedings of the National Academy of Sciences of the United States of America·Hong JiangK Lenhard Rudolph
Jul 10, 2010·Molecular & Cellular Proteomics : MCP·David M GoodPhilippe Schmitt-Kopplin
Feb 20, 2009·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Jens DrubeJochen H H Ehrich
Jun 26, 2009·QJM : Monthly Journal of the Association of Physicians·K BramhamA J Thompson
Dec 2, 2009·Transplantation·Luis F QuintanaJosep M Campistol
Aug 28, 2009·Seminars in Dialysis·Harald MischakJoachim Jankowski
Oct 27, 2010·PloS One·Alaa AlkhalafUNKNOWN PREDICTIONS Group
May 23, 2012·PloS One·Jesse DawsonWilliam Mullen
May 2, 2008·Journal of the American Society of Nephrology : JASN·Kasper RossingUNKNOWN PREDICTIONS Network
Apr 1, 2014·Clinical and Translational Medicine·Maria FrantziAgnieszka Latosinska
Jan 1, 2014·Journal of Proteomics·Maria FrantziAxel S Merseburger
May 24, 2014·Mass Spectrometry Reviews·Anthonius A M HeemskerkOleg A Mayboroda
Jun 15, 2007·Journal of Chromatography. a·Rob HaselbergGovert W Somsen
Jan 9, 2010·Proteomics. Clinical Applications·Bruce A JulianJan Novak
Apr 15, 2008·Electrophoresis·Miguel HerreroAlejandro Cifuentes
Feb 28, 2007·Journal of Mass Spectrometry : JMS
Mar 7, 2008·Proteomics. Clinical Applications·Dan TheodorescuHarald Mischak

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